Production of High Ethanol Concentration from Raw Corn Flour Using Rhizopus sp.and Saccharomyces cerevisiae

Abstract:

A novel raw-starch-digesting glucoamylase producer，Rhizopus sp.W-08，was used in a novel fermentation system of solid-state followed by submerged，and high enzyme activity of 72 IU/mL was obtained.In the following simultaneous saccharification and fermentation process，Saccharomyces cerevisiae Z-06 directly converted raw corn flour to ethanol with the concentration of 21 % (V/V) at 30℃ after 48h.The conversion efficiency of raw corn flour to ethanol was 94.5 % of the theoretical ethanol yield.