Expression and Enzymatic Characterization of Thermus thermophilus α-glucosidase Gene

Abstract:

A α-glucosidase gene (hbg) was amplified with PCR from the total DNA of Thermus thermophilus and linked with pGEM-T vector.Hbg gene was inserted into the expression vector pET-28a(+) and transformed into Escherichia coli BL21(DE3) with electroporation，finally the recombinant strain which could efficently secret recombinant α-glucosidase was obtained.Induced by IPTG，the expression products of hbg gene analysed by SDS-PAGE had a molecular mass of 59kD.The optimum temperature and optimum pH of the recombinant expression α-glucosidase were 95℃ and 5.0 respectively.