Study on the Breeding of Histidine Producing Mutant and Its Properties

Abstract:

A L-histidine producing mutant was derived from Corynebacterium glutamicum HZ4221（TRA^RDCP^RAMTRhistidase^-）by means of mutagenesis with N-methy-N′-nitro- N-nitrosoguanidine（NTG）.Contrast to original strain，the amount of histidine accumulation reached to a level of 5.31g/L in a medium containing 80g/Lglucose and 30g/L ammonium sulfate after cultured for 72 hours; the transketolase activity reduced to a degree of 15.7%.The utilization of the carbon sources，genetic stability，effect of metal ions were also been investigated in this paper.