Study on Separation and Purification of Zwittermicin A and its Stability

Abstract:

Zwittermicin A was purified by ion exchange resin and HPLC from supernatants of Bacillus thuringiensis.subsp.kurstaki strain D1-23 cultivation.2.89mg pure Zwittermicin A was acquired ，proved by HPLC-MS.Results show that the optimized wash concentration of NH₄H₂PO₄ is 5mmol/L at first step.Next step CH₃COONH₄ concentration is 30 mmol/L，the gradient pH is 8.0～9.5.Totally 93% Zwittermicin A can be reserved with ion exchange resin.The temperature and pH stability experiments show the half life of Zwittermicin A is 48.22 minutes in 100℃，and it is more stable in lower pH in pH 2.0～12.0.