Cloning and Expression of Nsp14 Gene of SARS Coronavirus

Abstract:

The Nsp14 gene of SARS coronavirus was cloned and expressed.On the basis of the reported amino acid sequence of the Nsp14 gene of SARS coronavirus，the primers were designed，the gene was cloned from the cDNA segment of SARS coronavirus by PCR，The target fragment was digested with BamHI and XhoI. Nsp14 gene was inserted into the prokaryotic expression vector pET30a to construct recombinant vector pET30a-Exo.Then，the competent E.coli cells were transformed by the recombinant vector and induced by IPTG.The Nsp14 were obtained in the form of fusion protein.It is a promising candidate for further studies.