Comparison with Several Methods to Isolate Epiphytic Bacteria from Gracilaria lemaneiformis (Rhodophyta)

Abstract:

We used 4 methods,such as ultrasonic crush(UC),ultrasonic rinse(UR),whorl surge(WS)and rubbing(RU),to isolate epiphytic bacteria from red alga Gracilaria lemaneiformis.Then,we counted bacteria numbers,detected bacterial species,observed bacterial configuration and characteristic of cell wall.Compared with these methods and with different treatments in one method,the results were drawn:the UR and RU were inferior in all methods to isolate bacterial numbers and species,the UC and WS were better,especially,the treatment 30W 30s of UC was the best in experiment,which isolated 12 of 16 bacterial species,and got 1.75 10⁶cells per gram wet weight G.lemaneiformis.

Key words:Seaweed; Gracilaria lemaneiformi; Epiphytic bacteria; Isolation method