A Methodological Study on Rapid Identification and Isolation of Algicidal Bacteria

Abstract:

In order to increase the efficiency of algicidal bacteria isolation,The components of minimal medium for isolation of algicidal bacteria were tested for the effection growth of Microcystis aeruginosa DS and a modified minimal medium was established..The results indi- cated that glucose at the concentration of 0.1～0.4g/L in minimal medium inhibited the growth of Microcystis aeruginosa DS,and a modi- fied minimal medium was developed using sodium citrate instead of glucose as carbon sourse.On modified medium algicidal bacteria could be succesfully isolated directly from field sample in 1 week. Microcystis aeruginosa DS could grow ewll on modified medium and the algicidal materials were secreted into medium by bacteria and the growth medium could be directly used for algicidal test.

Key words:Algicidal bacteria; Microcystis; Glucose; Rapid identification