cDNA Cloning and Sequence Analysis of Human Bactericidal/Permeability-increasing Protein

Abstract:

Human bactericidal/permeability-increasing protein(hBPI)cDNA was amplified by reverse transcription(RT)and touchdown PCR(TD-PCR)from blood stem cells collected from healthy human of Uygur nationality in Xinjiang Uygur Autonomous Region of China, and then was subcloned into pEGFP-N1 vector,hBPI cDNA sequence consists of 1,464bp.Comparison with other 4 hBPI cDNA sequences registered in GenBank identified 99% homology in DNA sequence.However,there were two base substitutions(nucleotide 576G→C,nu- clotide 676A→G),one of which resulted in an amino acid (residue 185 Lys→Glu).

Key words:Human bactericidal/permeability-increasing protein; cDNA; TD-PCR; Gene cloning; DNA sequence analysis