Studies on Purification and Some Properties of Antifungal Compounds in ZK-I Fermentation

Abstract:

ZK-I belongs to Bacillus sp.and shown strong antagonistic abilities to Saccharomyces cerevisiae and several plant pathogens,such as phytophthora sp,and Fusarium oxysporum f.sp.vasinfectum etc.,in this paper,we reported the purification of the antifungal substances and some properities of antifungal compounds.After acid precipitation,silica gel chromatography,C₁₈ reversed-phase silica gel chromatogra- phy,we obtained four compounds and knew their peaks were all single by HPLC testing,Combined with MS and HPLC, we concluded that they are the homologous series of compounds,A is jiean-peptide A and the others were confirmed to the analog of Jiean-peptide A in struc-ture.

Key words:Antimicrobial peptides; Separation; Purification; C₁₈ reversed-phase silica gel