Direct Amplification of Target Sequences by Taq DNA Polymerise Using Double-stranded Rna as Templates

Abstract:

Taq DNA polymerase has the activities of DNA polymerase and RNA reverse transcriptase.This research used the Double-stran- ded RNAs were used as templates for direct PCR,making use of the characteristics of Taq DNA polymerase.PCR for target sequences of 277,369 and 987 bp from dsRNA templates were performed directly with Taq DNA polymerase.When the sizes of target sequences were 277 bp and 369 bp,they were amplified with dsRNA templates on the denaturing temperatures of 47.0℃,47.9℃,50.2℃,52.6℃, 54.9℃,56.7℃ respectively. The results suggested that target sequences can be amplified with dsRNA templates only using Taq DNA polymerase alone and it is more effective for amplification of smaller sequences.