Since the human infection with avian influenza that occurred in Hong Kong in 1997, avian influenza virus has become a major threat to human health and public health.
Objective
To perform the molecular epidemiological study on a human infection with H10N3 avian influenza.
Methods
Influenza virus subtyping was detected by real-time quantitative polymerase chain reaction (PCR) method. The virus genome sequencing was completed on the next-generation sequencing platform. Bioinformatics software such as Blasts and Mega 6.1 was used for sequence and phylogenetic analysis.
Results
In April 2021, a virus was isolated from patients with severe respiratory diseases, which was confirmed as H10N3 subtype avian influenza virus by nucleic acid detection and sequence analysis. A H10N3 subtype avian influenza virus was isolated from the farm product market near the patient's residence, which was highly homologous with the human isolate. The human isolate was a new gene recombinant H10N3 avian influenza virus, and its HA and NA combination first appeared in poultry in East China in 2019. However, its six internal genes came from H9N2 virus prevalent in poultry in southern China in recent years. The HA cleavage site of the virus contained one basic amino acid R, without insertion of multiple basic amino acids. In theory, it did not belong to highly pathogenic avian influenza virus. The amino acid residue at 228 of HA receptor binding site mutated from G to S, which theoretically enhanced the affinity with human SA-α-2, 6 receptor. No E627K mutation of PB2 protein was found, but the amino acid residue at site 591 mutated from Q to K, which theoretically enhanced the adaptability to human body and the pathogenicity.
Conclusion
This paper reported the molecular epidemiological characteristics of a case of human infection with H10N3 avian influenza virus, and revealed that live poultry market played an important role in gene recombination of avian influenza virus and opportunistic infection.
禽流感病毒H10N3亚型人感染avian influenza A virusH10N3 subtypehuman infection江苏省重大科技示范项目BE2017749江苏省重大科技示范项目(BE2017749)the Science and Technology Demonstration Project of Jiangsu ProvinceBE2017749This work was supported by the Science and Technology Demonstration Project of Jiangsu Province (BE2017749)
在GenBank和GISAID网站通过BLASTn软件分析测序获得的序列,初步确定毒株亚型及各片段最大核苷酸相似毒株,并下载参考病毒核苷酸序列进行系统进化分析。用MegAlign Program (DNASTAR)软件进行核苷酸和氨基酸序列比对。采用MEGA 6.0软件的邻近法构建病毒基因组进化树,序列比对采用ClustalX 1.83法[22]。
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