Tobacco black shank, induced by Phytophthora nicotianae, is one of the major soil-borne diseases that cause serious stem and root rot in flue-cured tobacco production.
Objective
Screening of strong antagonistic strains is the basis of biological control.
Methods
In this study, the dual culture assay was used to screen Closnostachys strains against P. nicotianae. Sixty-five strains of Closnostachys belonging to eight species were tested. According to their abilities of sporulation on colony of P. nicotianae and hyphal coilings produced surrounding hyphae, sporangiophores, and sporangia of the pathogen, the antagonism of Closnostachys was classified into four degrees: strong, moderate, weak, and absent.
Results
A total of 6 tested strains had strong antagonistic ability, 27 moderate antagonistic ability, 22 weak antagonistic ability, and 10 no antagonistic ability. The inhibition rates of Closnostachys strains tested to P. nicotianae were 20.0%−86.7%.
Conclusion
The strains tested with strong antagonistic ability were C. rosea 7901 and 11361, C. byssicola 5072, 6729, and 7507, and C. grammicospora 6730. Their potential as biocontrol resources needs further pot experiment confirmation.
枝穗霉属烟草疫霉菌丝缠绕产孢量抑制率对峙培养ClonostachysPhytophthora nicotianaehyphal coilingssporulation capacityinhibition ratesdual culture method国家自然科学基金31870012国家自然科学基金31750001国家自然科学基金31570022中国科学院前沿重点研究项目QYZDY-SSW-SMC029国家自然科学基金(31870012,31750001,31570022);中国科学院前沿重点研究项目(QYZDY-SSW-SMC029)National Natural Science Foundation of China31870012National Natural Science Foundation of China31750001National Natural Science Foundation of China31570022Key Research Program of Frontier Sciences of Chinese Academy of SciencesQYZDY-SSW-SMC029National Natural Science Foundation of China (31870012, 31750001, 31570022); Key Research Program of Frontier Sciences of Chinese Academy of Sciences (QYZDY-SSW-SMC029)
Clonostachys strains with strong antagonism against Phytophthora nicotianae.
Clonostachys (fine) close associated with PP24 (coarse); D–F: Hyphal coilings of Clonostachys (fine) surrounding hyphae of PP24 (coarse); G: Hyphae of Clonostachys surrounding sporangiophores and sporangia of PP24. Scale bars: 10 μm.
]]>中等拮抗能力菌株
Clonostachys strains with moderate antagonism against Phytophthora nicotianae.
C. rosea 4354 (left) vs. P. nicotianae PP24 (right) in 5th, 8th and 30th day; D−F: C. rosea 7173 (left) vs. P. nicotianae (right) in 5th, 8th and 30th day; G: Very few hyphal coilings of 4354 (fine) surrounding PP24 hyphae; H: Rich hyphae of 7173 (fine) gathering around hyphae of PP24; I: Hyphae of 7173 (fine) surrounding sporangiophore and sporangium of PP24; J: Hyphae of 7173 (fine) associated with PP24 hyphae. K: Normal hyphae and sporangium of PP24 in blank control. Scale bars in G−K: 10 μm.
]]>弱拮抗能力菌株
Clonostachys strains with weak antagonism against Phytophthora nicotianae.
C. rosea 11539 (left) vs. P. nicotianae (right) in 5th, 8th and 30th day; D−G: A few hyphal coilings of 11539 (fine) surrounding PP24; H: Normal hyphae and sporangium of PP24 in blank control. Scale bars in D−H: 10 μm.
]]>无拮抗能力菌株
Clonostachys strains lack antagonism against P. nicotianae.
C. kowhai 7114 (left) vs. P. nicotianae PP24 (right) in 5th, 8th and 30th day; D−F: Without hyphal coilings of 7114 (fine) surrounding PP24. Scale bars in D−F: 10 μm.
]]>讨论
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