基于昆虫肠道纤维素降解菌领域的文献计量分析

Bibliometric analysis in the field of cellulose-degrading bacteria in insect gut

摘要:

【背景】近年来，昆虫肠道纤维素降解菌领域逐渐引起了学者们的关注。尽管相关科研著作逐年增加，但目前仍然缺乏对现有文献的系统梳理和可视化分析。【目的】探究昆虫肠道纤维素降解菌领域的研究现状与发展方向，为后续该领域的研究提供科学基础。【方法】以1995–2023年在Web of Science (WOS)核心合集中的193篇论文信息，对昆虫肠道纤维素降解菌研究领域的发文量、关键词、宿主昆虫、研究方法、纤维素降解菌等进行文献计量学分析。【结果】昆虫肠道纤维素降解菌年发文量总体呈现增长趋势。收录文献最多的期刊是Frontiers in Microbiology，Microbial Ecology总被引频次与篇均被引次数皆为最高。中国发文量位居第二，但篇均被引频次远低于日本、英格兰及德国等6个国家(地区)。统计到49种宿主昆虫，以农林害虫为主，占比77.97%，其次白蚁等财产害虫，占11.86%，其中鞘翅目受到多年持续关注。统计到昆虫肠道纤维素降解菌135种，涉及芽孢杆菌属(Bacillus)、假单胞菌属(Pseudomonas)、克雷伯菌属(Klebsiella)等63个细菌属，枝孢菌属(Cladosporium)、胶头霉属(Gliocephalotrichum)、青霉菌属(Penicillium)等14个真菌属。羧甲基纤维素是最多的供试纤维素，刚果红水解圈法是最常见的纤维素降解功能验证方法。传统分离培养技术在此领域一直发挥关键作用，近年来也在不断引入16S rRNA基因测序等技术进行多样性和分子机制研究。【结论】本研究对昆虫肠道纤维素降解菌领域现有文献进行了计量分析，有助于加深对本领域的整体认识，为后续研究开展提供基础依据。

Abstract:

[Background] Cellulose-degrading microbes in the insect gut have gradually garnered attention from scholars. Despite the increasing research papers, systematic reviews and visual analyses of these publications remain scarce. [Objective] To explore the research status and development direction and provide a basis for the subsequent research on cellulose-degrading microbes in the insect gut. [Methods] We conducted a bibliometric analysis of 193 papers about the cellulose-degrading microbes in the insect gut that were retrieved from the Web of Science Core Collection with the time interval of 1995 to 2023. The analysis encompassed various aspects, including publication counts, keywords, host insects, research methods, and microbial species. [Results] The annual publications of cellulose-degrading microbes in the insect gut generally showed an increasing trend. Frontiers in Microbiology had the largest publication count, and Microbial Ecology maintained the highest total cites and the highest average cited frequency per paper. China ranked second in the world in terms of publication count, whereas its average cited frequency per paper fell below that of six countries (regions), including Japan, the UK, and Germany. A total of 49 host insect species were counted, of which agricultural and forestry pests accounted for 77.97%, followed by property pests such as termites (11.86%), and Coleoptera had received increasing attention for many years. A total of 135 cellulose-degrading microbial species in the insect gut were involved, belonging to 63 bacterial genera (such as Bacillus, Pseudomonas, and Klebsiella) and 14 fungal genera (such as Cladosporium, Gliocephalotrichum, and Penicillium). Carboxymethyl cellulose was the most commonly used test cellulose, and the Congo red hydrolysis circle method was the most common method for verifying the function of cellulose degradation. The conventional isolation and culture method played a key role in this field, and 16S rRNA gene sequencing was increasingly introduced for the research on microbial diversity and molecular mechanism in recent years. [Conclusion] We conduct a bibliometric analysis of the publications about the cellulose-degrading microbes in the insect gut, which helps to deepen the understanding and provide a basis for subsequent research in this field.