Abstract:Microbial Physiology is a basic course for the undergraduates majoring in microbiology. With the advances of science and technology, the research field of Microbial Physiology is expanding. Accordingly, the teaching contents and mode of Microbial Physiology should be updated to reflect the state-of-the-art research achievements and adapt to the requirements of agricultural universities and colleges. Taking the teaching of myxobacteria as an example, we built the teaching and evaluation system composed of interest guidance, theory impartation, social practice, and comprehensive evaluation by two years of course development. Furthermore, we organized the field investigation and discussion on the topic of myxobacteria and agricultural applications. The teaching content innovation and teaching mode exploration of Microbial Physiology by combining the basic theory with agricultural practice improves the ability of undergraduates to think independently and apply what they have learned, providing support for training high-quality scientific and technological talents in agriculture.