芸薹根肿菌生理小种鉴定与检测方法研究进展

Research progress in pathotype identification and detection methods of Plasmodiophora brassicae Woronin

摘要:

根肿病是由芸薹根肿菌(Plasmodiophora brassicae Woronin)引起的全球性土传病害，对十字花科芸薹属作物的产量和质量构成了严重的威胁。根肿菌为专性活体寄生菌，具有强烈的生理小种分化现象，因此，对不同地区的生理小种类型进行评估，有利于芸薹属作物的抗性育种及作物栽培的整体规划。精确评估土壤中芸薹根肿菌休眠孢子的数量，有助于准确预测田块中根肿病发生的风险水平，可为后续防治方法的选择和防治效果的评估奠定基础。本文对芸薹根肿菌生理小种的鉴定以及土壤中根肿菌休眠孢子的检测和定量方法进行了简要综述，同时提出了一种有望应用于根肿菌生理小种研究的单细胞测序技术，并对重组酶聚合酶扩增技术(recombinase polymerase amplification, RPA)在根肿菌检测与定量方面的可行性进行了分析。本文旨在为根肿病的高效防控提供理论依据和方法参考，同时为根肿菌鉴定与检测方法的研究提供新的研究思路和解决方案。

Abstract:

Clubroot caused by Plasmodiophora brassicae Woronin is a globally prevalent soil-borne disease posing a severe threat to the yield and quality of Brassica crops. Due to the violent physiological differentiation and biotrophic characteristics exhibited by P. brassicae, evaluating the pathotypes of P. brassicae in different regions is crucial for breeding Brassica crops with disease resistance and planning the crop cultivation pattern. Accurately assessing the number of P. brassicae resting spores in the soil is instrumental in predicting the risk levels of clubroot in the fields, laying a foundation for subsequent selection of control methods and evaluation of their effectiveness. This paper briefs the methods for identifying P. brassicae pathotypes and detecting and quantifying the resting spores in the soil. Additionally, we propose a single-cell sequencing technique with potential application in the study of P. brassicae pathotypes and analyze the feasibility of recombinase polymerase amplification (RPA) in the detection and quantification of P. brassicae. The aim of this paper is to offer a theoretical foundation and methodological reference for the effective control of clubroot while providing new insights and solutions for the identification and detection of P. brassicae.