解脂耶氏酵母合成萜类化合物的研究进展

Research progress in the synthesis of terpenoids from Yarrowia lipolytica

摘要:

解脂耶氏酵母(Yarrowia lipolytica)是一种遗传背景清晰、基因编辑工具完备的非常规油脂酵母。萜类化合物是以异戊二烯为基本结构单元，广泛存在自然界的天然次级代谢产物。近年来随着合成生物学的高速发展，代谢工程改造解脂耶氏酵母合成萜类化合物越来越受到人们的关注。本文从增强前体供应、亚细胞器合成和碳源利用等方面对代谢工程改造解脂耶氏酵母合成萜类化合物进行综述，并对未来可能的研究方向进行展望，为后续研究工作提供参考。

Abstract:

Yarrowia lipolytica is a species of unconventional lipid-producing yeast with a well-defined genetic background and a comprehensive suite of gene editing tools. Terpenoids are natural secondary metabolites with isoprene as the basic structural unit and are ubiquitous in nature. In recent years, with the rapid development of synthetic biology, the synthesis of terpenoids by metabolic engineering has attracted increasing attention. In this paper, we reviewed the metabolic engineering in Y. lipolytica for the synthesis of terpenoids from enhanced precursor supply, subcellular organelle synthesis, and carbon source utilization. In addition, this paper envisions the potential research directions, with a view to providing guidance for the following research.