禾谷炭疽菌Velvet蛋白CgrVosA的生物学功能

Biological function of a Velvet protein CgrVosA in Colletotrichum graminicola

摘要:

【背景】禾谷炭疽菌(Colletotrichum graminicola)是侵染玉米的主要病原菌之一，每年造成严重的经济损失，直接威胁全球粮食安全。Velvet蛋白是真菌特有的一种保守性功能蛋白，它们在调控真菌生长及发育过程中发挥着重要作用。【目的】从禾谷炭疽菌中鉴定Velvet蛋白CgrVosA，并分析其生物学功能。【方法】利用同源重组的方法构建禾谷炭疽菌CgrvosA敲除突变株和互补菌株，通过测定其营养生长、胁迫因子响应、分生孢子产量及萌发、致病性等明确其功能。【结果】CgrvosA编码一个含有425个氨基酸的蛋白，有一个典型的Velvet蛋白结构域。与野生型菌株相比，敲除突变株ΔCgrvosA生长速度减慢，并且对NaCl和H₂O₂敏感；ΔCgrvosA卵圆形分生孢子的产量和萌发率显著降低，并且形态明显大于野生型菌株。【结论】禾谷炭疽菌Velvet蛋白CgrVosA参与调控菌株营养生长、对高渗和氧化应激的响应、卵圆形分生孢子的产生和萌发等过程。

Abstract:

[Background] Colletotrichum graminicola, one of the main pathogens infecting maize, causes serious economic losses and directly threatens the global food security every year. Velvet proteins are fungal unique proteins with conserved functions, playing a role in regulating the growth and development of fungi. [Objective] To study the biological functions of a Velvet protein CgrVosA identified from C.graminicola. [Methods] The CgrvosA-knockout mutant and complementary strain were constructed by homologous recombination. The vegetative growth, stress response, conidial yield, germination, and pathogenicity of the strains were determined and compared to reveal the functions of CgrVosA. [Results] CgrvosA encoded a 425-residue protein with a typical Velvet protein domain. Compared with the wild type, ΔCgrvosA exhibited slow growth, high sensitivity to NaCl and H₂O₂, enlarged oval conidia, and reduced yield and germination rate of oval conidia. [Conclusion] The Velvet protein CgrVosA is involved in the regulation of vegetative growth, response to hyperosmotic and oxidative stress, and oval conidial production and germination of C.graminicola.