基于Web of Science的环境领域中VBNC态细菌研究的文献计量学分析

Web of Science-based bibliometric analysis of VBNC state bacteria in environmental sciences

摘要:

【背景】活的但不可培养(viable but non-culturable, VBNC)状态是细菌应对不利环境压力的适应性反应，VBNC在医学和食品领域已经备受关注，但在环境领域中近10年才开始受到重视。目前，采用文献计量学对环境领域VBNC态细菌的研究尚未见报道。【目的】调查国内外环境领域VBNC态细菌的研究现状，归纳总结其研究热点、趋势与不足，旨在为环境领域VBNC态细菌的研究提供借鉴。【方法】采用Citespace、基于R语言的Bibliometrix工具包和VOSviewer对Web of science核心合集数据库中1994–2023年发表的环境领域VBNC态细菌文献(416篇)进行统计与可视化分析。【结果】近30年来，环境领域VBNC态细菌研究发文数量呈线性增长(R²=0.917)，中国和美国主导了该领域的研究，欧美科研机构较中国科研机构合作更密切，Oliver DJ、Yu X是该领域发文最多的作者。研究热点集中于饮用水消毒过程中VBNC态细菌的灭活及污染物处理过程中VBNC态功能菌的复苏。【结论】环境领域VBNC态细菌的研究主要围绕VBNC的形成、灭活及复苏开展，但VBNC的灭活技术和复苏方法仍十分有限，如何提高饮用水中VBNC的灭活效率和强化污染物微生物修复过程中VBNC的复苏是环境领域VBNC态细菌今后研究的发展方向。

Abstract:

[Background] Viable but non-culturable (VBNC) bacteria with adaptive responses to unfavorable environmental conditions have been extensively studied in the medical and food fields. However, environmental scientists have not paid attention to these bacteria until the last decade. Furthermore, few bibliometric studies in environmental sciences have been conducted regarding VBNC state bacteria. [Objective] The study investigated the research status of VBNC state bacteria in environmental sciences and summarized the research hotspots, trends, and shortcomings, aiming to provide references for the research in this field. [Methods] CiteSpace, Bibliometrix (a R package), and VOSviewer were used to statistically and visually analyze 416 available articles on VBNC state bacteria in environmental sciences that were published from 1994 to 2023 in the Web of Science Core Collection. [Results] In the last 30 years, the number of articles on VBNC state bacteria in environmental sciences showed linear growth (R²=0.917). China and the United States led the research in this field. European and American research institutions embraced close cooperation, while Chinese research institutions mainly cooperated domestically and worked independently. Oliver DJ and Yu X were the authors with the highest number of articles in this field. The research hotspots in this field included the inactivation of VBNC state bacteria in the disinfection of drinking water and the resuscitation of VBNC state bacteria in the treatment of pollutants. [Conclusion] The available studies about VBNC state bacteria in environmental sciences have mainly focused on the formation, inactivation, and resuscitation of VBNC state bacteria, while the inactivation and resuscitation methods remain to be improved. How to improve the inactivation efficiency of VBNC state bacteria in drinking water and enhance the resuscitation of VBNC state bacteria during microbial remediation of pollutants deserves further research.