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一株耐烟碱木质素降解菌的筛选及其对烟梗的降解效果
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贵州省烟草公司遵义市公司科技项目(2022XM05)


A lignin-degrading strain with nicotine tolerance: screening and the degradation effect on tobacco stems
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    摘要:

    【背景】在烟草废弃物中,木质素是环境微生物降解烟梗或其他秸秆木质纤维素的主要屏障,同时,烟梗中含有的烟碱使大部分已有报道的木质素降解微生物无法生存。【目的】筛选出对烟梗降解作用较强的菌株,为解决烟梗难降解而长时间堆积的问题和增加其工业可用性提供参考。【方法】以牛粪为材料,采用苯胺蓝褪色法以及愈创木酚显色法进行木质素降解优势菌株的筛选,并对降解菌进行烟碱耐受性测试,同时验证其对烟梗的降解效果。【结果】筛选出的耐烟碱木质素优势降解菌YP-2023-9为肠杆菌属(Enterobacter)菌株,对不超过0.60%的烟碱耐受,能产生木质素过氧化物酶Lip和锰过氧化物酶Mnp,促进烟梗木质素降解,在产酶培养基中,分别在第5天和第1天达到最大值为309.68 U/mL和21.76 U/mL。扫描电镜显示,该菌主要促进了烟梗表面木质素和半纤维素降解,对烟梗细胞壁有明显的破坏作用。30 ℃条件下,接种菌株YP-2023-9 15 d后烟梗降解率为42.42%,半纤维素和木质素的相对降解率分别为13.17%和33.12%。【结论】菌株YP-2023-9在降解烟梗和烟草废弃物方面具有很好的应用潜力。

    Abstract:

    [Background] The lignin in tobacco waste acts as a primary obstacle for environmental microorganisms to degrade lignocellulose in tobacco stems or other straw. Additionally, the nicotine in tobacco stem threatens the survival of most reported lignin-degrading microorganisms. [Objective] To screening the strains with strong degrading effects on tobacco stems, so as to solve the problem of long-term accumulation of tobacco stems which are difficult to degrade and enhance the industrial availability of tobacco stems. [Methods] The aniline blue fading method and the guaiacol assay were employed to screen out the dominant strains capable of degrading lignin from cattle manure. The nicotine tolerance of the strains was tested, and the degradation effects of the strains on tobacco stems were verified. [Results] The lignin-degrading strain YP-2023-9 screened belonged to Enterobacter and showed tolerance to nicotine with the concentration≤0.60%. The strain produced lignin peroxidase and manganese peroxidase, thereby promoting lignin degradation in tobacco stems. The maximum levels of the two enzymes reached 309.68 U/mL on day 5 and 21.76 U/mL on day 1, respectively. Scanning electron microscopy demonstrated that the strain primarily facilitated the degradation of lignin and hemicellulose on the tobacco stem surface, causing evident damage to the cell wall structure of tobacco stems. After incubation at 30 ℃ for 15 days, YP-2023-9 showed a degradation rate of 42.42% against tobacco stems, with the relative degradation rates of 13.17% and 33.12% against hemicellulose and lignin, respectively. [Conclusion] This strain exhibits promising potential for application in the degradation of both tobacco stems and associated waste.

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杨苹,刘京,周郑雄,王坡,谢世爽,黄莺. 一株耐烟碱木质素降解菌的筛选及其对烟梗的降解效果[J]. 微生物学通报, 2024, 51(6): 2182-2192

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历史
  • 收稿日期:2023-11-02
  • 最后修改日期:
  • 录用日期:2023-12-30
  • 在线发布日期: 2024-06-07
  • 出版日期: 2024-06-20