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低温纤维素降解细菌Duganella sp.的分离及降解能力解析
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国家自然科学基金区域创新发展联合基金(U22A20444);国家重点研发计划(2023YFD1500501);中央高校基本科研业务费专项基金(2572023CT08)


Isolation of a Duganella sp. of degrading cellulose bacterium at low temperatures and analysis of its degradation capacity
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    摘要:

    【背景】低温是寒区秸秆还田后微生物降解的主要限制因素之一。【目的】获得低温纤维素降解细菌以提高寒区秸秆资源化利用率低的问题。【方法】取冬季土壤样品进行富集,以羧甲基纤维素钠(sodium carboxymethyl cellulose, CMC-Na)为唯一碳源,采用稀释涂布等方法分离纯培养菌株,通过单因素试验及正交试验对菌株产酶条件进行优化。【结果】获得一株15 ℃条件下降解纤维素的细菌,初步归类为杜擀氏菌属(Duganella)。其最佳产酶条件为:培养温度15 ℃,初始pH值7.0,装液量50/100 mL,接种量12.5%,此时的CMC酶活最高为398.2 U/mL,比优化前提高了40.45%。菌株在最佳培养条件下对滤纸、水稻秸秆和玉米秸秆的降解率分别为19.24%、9.48%和7.30%。【结论】本研究为寒区秸秆降解微生物资源提供了新的菌种资源,同时为后续低温纤维素降解菌株的研究提供了数据参考。

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    [Background] Low temperatures are one of the main factors limiting microbial degradation of straw returned to the field in cold regions. [Objective] To obtain the bacterial strains capable of degrading cellulose at low temperatures to improve the straw reutilization in cold regions. [Methods] The soil samples were collected in winter for strain enrichment with sodium carboxymethyl cellulose (CMC-Na) as the sole carbon source. The pure culture of the strain was obtained by the dilution-plate coating method, and the enzyme production conditions of the strain were optimized by single factor and orthogonal tests. [Results] A cellulose-degrading strain was obtained at 15 ℃ and identified as Duganella sp. The optimal enzyme production conditions of the strain were incubation with the liquid loading volume of 50/100 mL and inoculum volume of 12.5% at 15 ℃ and initial pH 7.0, under which the highest CMC enzyme activity was 398.2 U/mL, 40.45% higher than that before optimization. The degradation rates of filter paper, rice straw, and maize straw by the strain were 19.24%, 9.48%, and 7.30%, respectively, under optimal culture conditions. [Conclusion] This study provides new strain resources for straw degrading microbial resources in cold regions, as well as data reference for subsequent research on low temperature cellulose-degrading strains.

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陈昭,王敬红,邓常宇,邹世杰,范寒雪,魏丹,孙宇峰,王伟东. 低温纤维素降解细菌Duganella sp.的分离及降解能力解析[J]. 微生物学通报, 2024, 51(6): 2158-2169

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历史
  • 收稿日期:2023-12-31
  • 最后修改日期:
  • 录用日期:2024-01-22
  • 在线发布日期: 2024-06-07
  • 出版日期: 2024-06-20