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贝莱斯芽孢杆菌(Bacillus velezensis) PZ-3吸附载体的筛选及对玉米根际土壤微生物多样性的影响
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国家重点研发计划(2023YFD1500501-01);黑龙江省揭榜挂帅科技攻关项目(2022ZXJ08B01);国家自然科学基金区域创新发展联合基金(U22A20444);中央高校基本科研业务费专项资金(2572023CT08);黑龙江八一农垦大学三纵计划(ZDZX202105)


The adsorption carrier of Bacillus velezensis PZ-3: screening and effects on microbial diversity in the rhizosphere soil of maize
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    摘要:

    【背景】载体类型对微生物肥料的应用效果和成本有显著影响。【目的】筛选吸附贝莱斯芽孢杆菌(Bacillus velezensis) PZ-3能力强的载体。【方法】以生物炭、腐殖酸、生物质灰渣、有机肥、菌糠和稻壳粉作为载体原料,以贝莱斯芽孢杆菌PZ-3为试验菌株与载体进行吸附试验,通过稀释涂布-菌落计数法,流式细胞术及玉米盆栽试验,筛选与目标菌株适配的载体,并探究其吸附目标菌株后对玉米根际土壤中微生物多样性的影响。【结果】腐殖酸+菌糠吸附目标菌株后在玉米根际土壤中定殖效果最佳,根际土壤中目标菌株的数量在第7天达到最高,为1.28×105 CFU/g,其相对丰度为59.15%;该处理与原始土壤相比,玉米的株高、茎粗、地上物质干重提升了28.51%、19.05%、48.65%;且该处理显著提升(p<0.05)玉米根际土壤中链霉菌属(Streptomyces)、假单胞菌属(Pseudomonas)的相对丰度,显著降低了索氏梭菌属(Paeniclostridium)、瓶毛壳属(Lophotrichus)、赤霉菌属(Gibberella)的相对丰度。【结论】腐殖酸+菌糠吸附贝莱斯芽孢杆菌PZ-3的能力强,联合配施对玉米促生效果明显,且显著提升玉米根际土壤中植物促生菌的相对丰度,降低植物病原真菌的相对丰度。

    Abstract:

    [Background] Carriers influences the effects and costs of microbial fertilizers. [Objective] To screen the vector with strong adsorption ability of Bacillus velezensis PZ-3. [Methods] We carried out adsorption experiments for B. velezensis PZ-3 with biochar, humic acid, biomass ash, organic fertilizer, mushroom bran, and rice husk powder as carriers. The carrier suitable for the target strain was screened by dilution coating-colony counting method, flow cytometry, and maize pot experiment. Then, we investigated the effects of the adsorbed target strain on the microbial diversity in the rhizosphere soil of maize. [Results] The best colonization effect was achieved in the rhizosphere soil of maize after the adsorption of the target strain by humic acid+mushroom bran. The abundance and number of the target strain in the rhizosphere soil of this treatment peaked at 1.28×105 CFU/g, with a relative abundance of 59.15%, on day 7. Compared with the control (original soil), this treatment increased the plant height, stem diameter, and aboveground dry weight of maize by 28.51%, 19.05%, and 48.65%, respectively. Moreover, this treatment increased the relative abundance of Streptomyces and Pseudomonas (P<0.05) and reduced the relative abundance of Paeniclostridium, Lophotrichus, and Gibberella in the rhizosphere soil of maize. [Conclusion] Humic acid combined with mushroom bran had a strong ability to adsorb B. velezensis PZ-3. The combination significantly promoted the growth of maize, increased the relative abundance of plant growth-promoting strains, and reduced the relative abundance of plant pathogenic fungi in the rhizosphere soil of maize.

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窦鑫,郭鹏博,张伟,王敬红,陈昭,洪艳华,孙冬梅,王伟东. 贝莱斯芽孢杆菌(Bacillus velezensis) PZ-3吸附载体的筛选及对玉米根际土壤微生物多样性的影响[J]. 微生物学通报, 2024, 51(6): 2013-2029

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  • 收稿日期:2024-01-01
  • 最后修改日期:
  • 录用日期:2024-03-25
  • 在线发布日期: 2024-06-07
  • 出版日期: 2024-06-20