植物内生菌研究方法

Advances in research methods for plant endophytes

摘要:

植物内生菌是植物微生态系统中不可或缺的组成部分，植物微生物类群的主要成员是内生菌，它们栖息在植物组织或组织间隙中，并且多数内生菌通过不同的途径协助植物生长。植物中普遍存在种类丰富且结构复杂的内生菌群落，有益内生菌具有促进植物生长、增强植物抗逆性、产生有利用价值次生代谢物的功能。本文通过对国内外内生菌资源研究方法的相关文献回顾，探讨植物内生菌与宿主植物的关系、内生菌多样性、分离和定殖的研究方法，探索内生菌资源对宿主植物的作用和相关机制，展望新技术在植物内生菌研究方面的趋势，为其在相关领域的应用提供理论参考。

Abstract:

Plant endophytes as indispensable components of plant microcosms are the major part of the plant microbial community. They inhabit plant tissues or tissue gaps, and most of them promote plant growth via different pathways. The plant endophyte community is characterized by diverse species and a complex structure. Beneficial endophytes can promote plant growth, enhance plant resistance, and produce valuable secondary metabolites. By reviewing the articles about the research methods for endophyte resources, we explored the relationship between plant endophytes and host plants, the diversity of endophytes, the research methods for isolation and colonization, and the roles and functioning mechanisms of endophytes in host plants. Furthermore, we make an outlook on the trend of new technologies in the research on plant endophytes, aiming to provide a theoretical reference for the application of plant endophytes.