Abstract:Microbiology Experiment is a foundation course for life sciences, food sciences, and other disciplines in colleges and universities. With strong practicality and applicability, this course plays an important role in the cultivation of innovative talents in biology. The current teaching of Microbiology Experiment in local universities face problems such as cramming teaching, dull content, low participation of students, and unreasonable assessment methods. Considering these problems and the requirements of engineering certification and first-class course initiative, we established a "123+2n" system for teaching reform and innovation. This system includes innovative reform of the teaching concept, teaching content, teaching methods, assessment methods, and evaluation methods. It aims to stimulate students' enthusiasm for learning, unleash their subjective initiative, and improve their innovation awareness and engineering practice ability, achieving the teaching goals of value shaping, knowledge impartment, and competence training. The results showed that this system significantly improved students' interest in learning, problem-solving abilities, and innovation abilities. This new system can provide reference for the teaching reform of experimental courses in local universities.