Abstract:Fermentation Process Design is a distinctive practical course offered by the College of Biology at Hunan University for the undergraduates majoring in biotechnology. This course aims to enhance students' practical skills and application abilities, encouraging them to apply their acquired knowledge of microbiology and fermentation engineering in practical settings such as fermentation factories. The traditional teaching of this course primarily relies on classroom lectures. However, due to the practical nature of topics such as operating fermentation production lines and designing fermentation equipment parameters, we found that simple classroom lectures were not effective in triggering students' enthusiasm and providing them with a clear understanding of the teaching content. Therefore, we reformed the teaching for this course by introducing the teaching model of "classroom+virtual simulation+factory", aiming to improve students' understanding of the knowledge about fermentation engineering and their practical and application abilities. This article introduces the current three-dimensional integrated model of "classroom+virtual simulation+factory" in the teaching of Fermentation Process Design and evaluates the teaching effectiveness with the feedback from students. We hope that our work will provide reference information for the teaching reform of colleges and universities.