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一株煤中有机钠脱除菌的分离、优化及其作用效果
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国家自然科学基金(22178245)


Isolation, optimization and effect of organic sodium-removing bacterium from coal
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    摘要:

    【背景】 针对煤燃烧过程中碱金属钠对燃煤设备和气化效率的影响,以及酸洗过程中产生酸性废水等问题。【目的】 提出利用微生物脱除煤中有机钠的新方法。【方法】 从内蒙古乌兰察布市察哈尔右翼前旗黄旗海分离、筛选一株脱除煤中有机钠的菌株,通过形态特征、生理生化特性以及16S rRNA基因序列分析对菌株进行鉴定,构建其系统发育树,通过单因素试验探究细菌最适的生长条件并绘制其生长曲线,确定煤中有机钠最佳的脱除条件,并在最佳条件下评估其脱除效果。【结果】 筛选到一株能够脱除煤中有机钠的菌株,经鉴定为水生鞘氨醇单胞菌。该菌株的最佳生长条件为:温度30 ℃、初始pH 8.0、接种量3%、装液量100 mL、摇床转速150 r/min、最佳碳源为葡萄糖(4 g/L)、最佳氮源为蛋白胨(13 g/L);煤中有机钠的最佳脱除条件为:初始pH 8.0、装液量80 mL、煤浆浓度5 g/L、接种量7%。该条件下菌株CSW-1对煤中有机钠的脱除率为23.79%。【结论】 首次提出细菌脱除煤中有机钠的新方法,为煤炭的高效清洁利用提供了新思路。

    Abstract:

    [Background] The alkali metal sodium affects coal burning equipment and gasification efficiency in the coal combustion process and acid wastewater is produced in the pickling process. [Objective] To propose a novel method for removing organic sodium from coal by microorganisms. [Methods] A bacterial strain, which was isolated and screened out from Huangqihai, Chahar Right Front Banner, Ulanqab, Inner Mongolia, was identified based on morphological, physiological, and biochemical characteristics and the 16S rRNA gene sequence, and a phylogenetic tree was constructed. The optimal growth conditions of the strain were investigated by single factor tests, and the growth curve was plotted. The optimal removal conditions of organic sodium in coal by the strain were determined, and the removal effect was evaluated under the optimal conditions. [Results] A strain that can remove organic sodium from coal was screened out and identified as Sphingomonas aquatilis. The strain showed the best growth performance in the medium with glucose (4 g/L) as the carbon source, peptone (13 g/L) as the nitrogen source, and initial pH 8.0 when it was cultured at 30℃ and 150 r/min with the inoculum quantity of 3% and liquid loading of 100 mL. The optimal removal conditions of organic sodium from coal were as follows:initial pH 8.0, liquid loading of 80 mL, coal slurry concentration of 5 g/L, and inoculum quantity of 7%. Under the conditions, the removal efficiency of organic sodium from coal by strain CSW-1 was 23.79%. [Conclusion] A novel method for removing organic sodium from coal by microorganisms is proposed for the first time, which provides a new idea for the efficient and clean utilization of coal.

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陈转明,温全宝,刘生玉,郭建英,滕青. 一株煤中有机钠脱除菌的分离、优化及其作用效果[J]. 微生物学通报, 2024, 51(5): 1550-1565

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  • 收稿日期:2023-09-20
  • 最后修改日期:
  • 录用日期:2023-12-22
  • 在线发布日期: 2024-05-09
  • 出版日期: 2024-05-20
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