重组酶聚合酶扩增技术的研究进展

Research progress on recombinase polymerase amplification

摘要:

重组酶聚合酶扩增(recombinase polymerase amplification, RPA)技术是一种利用重组酶(T4 UvsX)、单链DNA结合蛋白(T4 Gp32)和链置换DNA聚合酶(Bsu)等蛋白在恒温条件下扩增核酸的技术。由于其可以恒温、高效地扩增靶核酸片段，近几年在细菌、病毒、寄生虫、支原体、衣原体检测方面发挥着独特的作用，逐渐成为床旁检测(point-of-care testing, POCT)的重要技术。本文以RPA技术的原理为起点，对其临床应用、不足之处及改进方法进行阐述，以期为临床疾病的快速检测和诊断方面提供新思路。

Abstract:

Recombinase polymerase amplification (RPA) is a technology that utilizes proteins such as recombinase (T4 UvsX), single-stranded DNA binding protein (T4 Gp32), and strand replacement DNA polymerase (Bsu) to amplify target nucleic acids at constant temperatures. In recent years, RPA plays a unique role in the detection of bacteria, viruses, parasites, mycoplasma, and chlamydia for it can amplify the target nucleic acid fragment with constant temperature and high efficiency, it gradually becomes a key technology for point-of-care testing (POCT). This review takes the principle of RPA technology as a starting point, it describes the clinical application, shortcomings, and improvement of RPA, and RPA is expected to provide a new idea for the rapid detection and diagnosis of clinical diseases.