Abstract:Fermentation Engineering is a characteristic engineering course featuring extensive knowledge, rapid discipline development, and close integration with production. This course is designed to cultivate engineering talents with strong practical ability, innovative thinking, and engineer spirit. Focusing on the shortcomings and difficulties of the theoretical course in the training of engineering talents, we have established a teaching model of "three modules+two passes+three step progression" according to the gold class standard of being advanced, innovative, and challenging after four years of exploration on the teaching reform of Fermentation Engineering. In this model, the online and offline teaching content is re-organized, with the introduction of cutting-edge knowledge; an active learning environment is created to cultivate advanced thinking; contextualized project-based teaching method is employed to provide practical scenarios and challenging tasks; a multi-assessment model composed of students, teachers, and corporate (expert) judges is formed to stimulate the enthusiasm for learning; the forms of ideological and political education are enriched to improve the attraction of the course. The teaching reform has stimulated the teachers' enthusiasm for teaching and building rapport with students and improved the students' participation, experience, and initiative, with the formation of a distinctive feature of combining theory with practice and engineering. Furthermore, the reform has improved students' self-learning, collaborative ability, skills in solving complex engineering problems, and gaining sense, achieving the teaching goals.