Abstract:Microbiology Experiment is an important course of microbiology in institutions of higher education. This course aims to not only impart basic skills, verify and expand theories in microbiology, but also train senior undergraduates for graduation projects and further research work in their graduate education. To train the scientific research thinking of students who had mastered the basic experimental techniques of microbiology, we designed the class of bacterial identification based on physiological and biochemical tests in the form of a flipped classroom. The students were guided to review textbooks and papers before class and explain the experimental principles, expect experimental results, observe and correct the results, and analyze and discuss experimental results during class. The teaching form of flipped classroom enlivens the classroom atmosphere, enabling students to better access textbooks for preview before class. The analysis of experimental results and the revision of experimental conclusions have trained the scientific literacy of meticulous observation, scientific analysis, and rigorous conclusion drawing for the students.