Abstract:Microbiology stands as a foundational discipline in life sciences and plays a pivotal role in the biology curriculum of universities. However, the conventional microbiology laboratory teaching faces issues such as content fragmentation with a lack of inherent connections. These challenges hinder students to deeply comprehend and apply experimental skills and knowledge. Moreover, the conventional teaching fails to stimulate students' self-directed learning and exploratory spirit. To enhance the quality of microbiology laboratory teaching, we draw inspiration from project-based learning. With the introduction of Koch's postulates, a fundamental theory, as the goal, we improved the educational philosophy, teaching modes, teaching methods, and assessment system. Using silkworm and Bacillus thuringiensis as the experimental subjects, we reproduced and confirmed Koch's postulates, and probed into the cause-and-effect relationship between infectious diseases and pathogenic microorganisms. Our improved teaching approach aims to kindle students' passion for experimentation, refine their practical skills, nurture their critical thinking, and elevate their scientific literacy and innovative capabilities.