Abstract:The development of new agricultural sciences has put forward higher requirements for talent cultivation in higher agricultural colleges and universities and has set new standards for the development of core courses. Microbiology is a basic compulsory course offered for all the undergraduates majoring in agricultural and life sciences, providing strong support for cultivating students' innovation thinking and practical abilities. This article introduces the teaching reform practice of Microbiology in Jilin Agricultural University. The reform measures include improving the online classes, optimizing the teaching content, innovating the teaching reform concept, and reforming the evaluation of course performance. These measures helped to achieve the knowledge, ability, and emotional goals of talent cultivation in the context of new agricultural sciences and improved the teaching quality, providing a reference for the course development in higher agricultural colleges and universities.