Abstract:The traditional teaching of Experiments in Food Microbiology have problems such as the lack of inquiry experiments in teaching content, the neglect of student subjectivity in teaching methods, and the lack of process assessment. In view of these problems and according to the cultivation objectives of national first-class undergraduate specialty, we organized the teaching content involving 3 modules with a total of 8 units combining basic skill learning and inquiry experiments, established an "online + offline" mixed teaching method, and introduced a process assessment method at three levels of units, modules, and practical operations. This teaching reform has improved the depth and breadth of the course and promoted personalized learning. This paper introduces the reform of teaching system for Experiments in Food Microbiology and analyzes the challenges in the reform based on teaching practice, aiming to promote the construction of first-class undergraduate specialty and cultivate outstanding talents in food science.