金针菇几丁质合成酶基因家族鉴定及其表达分析

Characterization and expression analysis of chitin synthase family in Flammulina filiformis

摘要:

【背景】几丁质是真菌细胞壁的重要成分，由几丁质合成酶（chitin synthase，CS）催化合成。几丁质合成酶编码基因在大型食用真菌金针菇中的数量及表达规律尚不明确。【目的】探究几丁质合成酶基因在金针菇中存在的数量及其在子实体不同发育时期的表达规律，为其在大型真菌子实体生长发育过程中的功能研究提供基础。【方法】基于已有的金针菇菌株L11基因组数据，结合NCBI其他真菌CS序列鉴定金针菇中几丁质合成酶编码基因的数量，并对其进行生物信息学分析。进一步根据金针菇F19转录组数据以及实时荧光定量PCR （RT-qPCR）技术分析金针菇CS基因家族的表达规律。【结果】在金针菇单核体菌株L11的基因组中鉴定到9个几丁质合成酶基因，系统发育分析表明它们在子实体发育过程中的表达模式可分为4类（皮尔森相关系数=0.85）。【结论】金针菇CS基因家族表达模式在金针菇不同生长发育时期均存在差异，可能参与了子实体发育不同时期和组织的形态建成。

Abstract:

[Background] In fungi, chitin is an important component of the cell wall and synthesized under the catalysis of chitin synthase (CS). Its presence and expression pattern in Flammulina filiformis remain unclear. [Objective] To investigate the number of CS genes and their expression patterns in F. filiformis at different development stages, so as to provide a basis for revealing the roles of these genes in the growth and development of macrofungi. [Methods] The genome data of F. filiformis L11 and the CS sequences of other fungi in the NCBI database were employed to identify the CS genes in F. filiformis. Bioinformatics analysis and phylogenetic analysis were then carried out for these genes. Furthermore, the transcriptome data of F. filiformis F19 and RT-qPCR were employed to analyze the expression patterns of the CS gene family in F. filiformis. [Results] In the genome of the single-spore strain L11 of F. filiformis, nine chitin synthase genes were identified. Phylogenetic analysis revealed that they belong to four distinct classes. The expression patterns of these genes during fruiting body development can be classified into four categories (Pearson correlation coefficient=0.85). [Conclusion] The expression patterns of the CS gene family in F. filiformis show variations at different growth and developmental stages, indicating their potential involvement in morphogenesis of different tissues and developmental stages in the fruiting body formation of F. filiformis.