暹罗炭疽菌同源异型盒转录因子CsHtf1的生物学功能

Biological function of a homeobox transcription factor CsHtf1 in Colletotrichum siamense

摘要:

【背景】暹罗炭疽菌(Colletotrichum siamense)是橡胶炭疽病害的主要致病菌，严重制约着天然橡胶产量。在植物致病真菌中广泛存在同源异型盒转录因子，其参与调控真菌无性生殖、侵染和代谢等诸多方面。【目的】明确在暹罗炭疽菌中鉴定的一个同源异型盒转录因子CsHtf1的生物学功能。【方法】利用同源重组的方法获得Cshtf1基因的敲除突变株，并对其营养生长、孢子产生和致病性等表型进行分析。【结果】Cshtf1基因编码600个氨基酸且含有1个HOX结构域；与野生型相比，Cshtf1敲除突变株营养生长和致病性无显著差异，而突变株分生孢子产量显著降低且黑色素产量增加。【结论】CsHtf1参与调控暹罗炭疽菌的分生孢子及黑色素产生。

Abstract:

[Background] Colletotrichum siamense was the main pathogen of rubber anthracnose, which seriously restricted the yield of natural rubber. Homeobox transcription factors were widely found in plant-pathogenic fungi and involved in asexual reproduction, infection, and metabolism of fungi. [Objective] To determine the biological function of a homeobox transcription factor CsHtf1 identified in Colletotrichum siamense. [Methods] The gene-knockout mutant of Cshtf1 was obtained by homologous recombination, and its phenotypes such as vegetative growth, conidial production, and pathogenicity were analyzed. [Results] The Cshtf1 gene encoded 600 amino acids and contained a HOX domain. Compared with the wild type, the Cshtf1 knockout mutant showed no significant difference in vegetative growth and pathogenicity, whereas the conidial production of the mutant was significantly reduced, and the production of melanin increased. [Conclusion] CsHtf1 was involved in the regulation of conidiation and melanin production in Colletotrichum siamense.