Abstract:“Environmental Engineering Microbiology” is a fundamental course in the field of environmental engineering. However, due to the abstract concepts and extensive content of Environmental Microbiology, most students have difficulty concentrating in class and struggle to understand and apply topics such as biological oxidation processes and the role of microorganisms in environmental engineering. As a result, the teaching of effectiveness is undesirable. In order to improve students’ learning efficiency and teachers’ teaching experience, a blended teaching reform and practice of “Environmental Engineering Microbiology” was carried out based on the Chaoxing Fan Ya online learning platform. The practical results show that the reformed course has stimulated students’ intrinsic motivation for learning, developed their ability for self-study and continuous learning, and achieved positive effects in terms of knowledge, skills, and overall qualities. It has effectively enhanced students’ professional abilities and comprehensive qualities. At the same time, the teaching methods, teaching abilities, teaching experience, and information technology of the course teachers have been improved and enhanced, strengthening the practice of course innovation and improving the teaching quality of Environmental Engineering courses.