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从宏基因组推测扎龙湿地未培养甲烷古菌Rice Cluster II的代谢途径与可能的盐碱适应性
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国家自然科学基金(92251302,32070061,31971468)


Metagenomic analysis of metabolic pathways and possible saline-alkaline adaptation of uncultured methanogens of Rice Cluster Ⅱ in Zhalong wetland
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  • LI Huiju

    LI Huiju

    Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing 100044, China;Beijing Energy Conservation & Sustainable Urban and Rural Development Provincial and Ministry Co-construction Collaboration Innovation Center, Beijing University of Civil Engineering and Architecture, Beijing 100044, China
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  • LI Lingyan

    LI Lingyan

    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
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  • LIANG Hong

    LIANG Hong

    Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing 100044, China;Beijing Energy Conservation & Sustainable Urban and Rural Development Provincial and Ministry Co-construction Collaboration Innovation Center, Beijing University of Civil Engineering and Architecture, Beijing 100044, China
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  • GAO Dawen

    GAO Dawen

    Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing 100044, China;Beijing Energy Conservation & Sustainable Urban and Rural Development Provincial and Ministry Co-construction Collaboration Innovation Center, Beijing University of Civil Engineering and Architecture, Beijing 100044, China
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  • DONG Xiuzhu

    DONG Xiuzhu

    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
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    摘要:

    【背景】湿地是重要的甲烷排放源,因为其中栖息着各种产甲烷古菌。已知未培养甲烷古菌Rice Cluster II (RCII)类群广泛分布于低温酸性和北方泥炭藓湿地、淡水湿地及草本沼泽等环境,但它们在低温盐碱湿地中的分布及代谢途径尚未知。【目的】分析扎龙盐碱湿地未培养甲烷古菌RCII类群的多样性、推测产甲烷代谢途径及其潜在的盐碱适应机制。【方法】16S rRNA基因扩增子测序分析扎龙湿地土壤中甲烷古菌群组成;构建16S rRNA基因克隆文库分析扎龙湿地土壤RCII的多样性;宏基因组分析推测RCII古菌编码的产甲烷途径及与耐盐碱相关物质的合成基因。【结果】16S rRNA基因高通量测序发现未培养甲烷古菌的RCII类群占扎龙盐碱湿地总甲烷古菌的13.280%±0.019%;系统发育学分析表明该湿地的RCII由3个分支组成;宏基因组分析组装了2个优势的未培养RCII的基因组,均含完整的氢还原二氧化碳产甲烷途径的基因,并编码海藻糖的转运与合成基因。【结论】扎龙盐碱湿地土壤富含未培养RCII甲烷古菌,推测它们通过氢还原二氧化碳产甲烷,利用细胞内高的海藻糖适应盐碱环境。

    Abstract:

    [Background] Wetlands harbobring a variety of methane-producing archaea emit ample methane. The uncultured methanogens, Rice Cluster II (RCII), exist widely in cold acidic peatlands, freshwater lake wetlands, minerotrophic fens, and northern peat moss. However, the distribution and metabolic pathways of RCII in the low-temperature saline-alkaline wetlands remain unknown. [Objective] To investigate the diversity and infer the methanogenic pathways and potential adaptation mechanisms of RCII in saline-alkaline Zhalong wetland. [Methods] High-throughput sequencing of the 16S rRNA genes in the top layer of soil was performed to reveal the composition of methanogenic archaea. We established the 16S rRNA gene clone library to analyze the phylogenetic clusters of RCII. We then conducted metagenomic analysis to infer the methanogenic pathways and the possible saline-alkaline adaption mechanisms of the RCII in Zhalong wetland.[Results] The uncultured RCII methanogens accounted for 13.280%±0.019% of the total methanogenic archaea in Zhalong wetland and were clustered into three phylogenetic groups. Two metagenome-assembled genomes of RCII possessed the complete suite of genes involved in methanogenesis via reduction of carbon dioxide. Furthermore, the two RCII genomes contained the genes associated with the transport and synthesis of trehalose. [Conclusion] Abundant uncultured RCII methanogens inhabit the saline-alkaline Zhalong wetland, and they can produce methane by reducing carbon dioxide and use the compatible solute trehalose to adapt to the saline-alkaline environment.

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李彗菊,李凌燕,梁红,高大文,东秀珠. 从宏基因组推测扎龙湿地未培养甲烷古菌Rice Cluster II的代谢途径与可能的盐碱适应性[J]. 微生物学通报, 2023, 50(10): 4343-4356

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  • 收稿日期:2023-03-25
  • 录用日期:2023-05-12
  • 在线发布日期: 2023-10-07
  • 出版日期: 2023-10-20
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