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噬菌体鸡尾酒对猕猴桃溃疡病的田间防治效果及叶际细菌群落结构的影响
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中央高校基本科研业务费专项资金项目(2021ZY64)


Phage cocktail inhibits bacterial canker of kiwifruit in the field and affects the phyllospheric bacterial community
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    摘要:

    【背景】噬菌体鸡尾酒可作为一种杀灭猕猴桃溃疡病病原菌(Pseudomonas syringae pv. actinidiae, Psa)的生物制剂,但关于噬菌体鸡尾酒在田间的防治效果和对猕猴桃植株叶际内生细菌群落结构影响的研究依然较少。【目的】探究噬菌体鸡尾酒在田间防控猕猴桃溃疡病的效果,以及噬菌体鸡尾酒对猕猴桃茎内叶际细菌微生态的影响。【方法】使用猕猴桃溃疡病病原菌感染健康植株,对比施用噬菌体鸡尾酒和传统铜制剂后溃疡病的发病情况,利用高通量测序技术分析猕猴桃叶际内生细菌群落结构的变化。【结果】与铜制剂相比,噬菌体鸡尾酒可更有效地控制猕猴桃溃疡病,改变叶际细菌群落的丰富度与多样性,增强群落结构的稳定性,改善群落物种功能基因丰度情况,一定程度使叶际细菌群落恢复至健康状态。【结论】噬菌体鸡尾酒在杀灭病原菌的同时具有良好的微生态调节功能,在猕猴桃溃疡病的生物防治中具有巨大的应用潜力。

    Abstract:

    [Background] Phage cocktails have been reported as biocontrol agent against the kiwifruit canker pathogen Pseudomonas syringae pv. actinidiae (Psa). However, little is known about the control effect of phage cocktail in the field and the effect of phage cocktail on the phyllospheric bacterial community of kiwifruit plants. [Objective] To explore the biocontrol effect of phage cocktail against kiwifruit canker in the field, and reveal the effect of the cocktail on the microecology of endophytic bacteria in the stem phyllosphere of kiwifruit. [Methods] The healthy kiwifruit plants were infected with Psa, and the incidence of bacterial canker after application of phage cocktail or copper preparation was recorded. The changes in the community structure of bacteria in kiwifruit phyllosphere were analyzed by high-throughput sequencing. [Results] Compared with the copper preparation, the phage cocktail significantly reduced the incidence of bacterial canker, changed the richness and diversity of bacteria in the phyllosphere, enhanced the stability of bacterial community, and improved the abundance of functional genes, thus restoring the bacterial community to the healthy state. [Conclusion] Phage cocktails exert the role of microecological regulation while killing pathogenic bacteria, demonstrating great application potential in the biocontrol of kiwifruit canker caused by Psa.

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胡冉,刘梦娇,许小含,何晓青,金一. 噬菌体鸡尾酒对猕猴桃溃疡病的田间防治效果及叶际细菌群落结构的影响[J]. 微生物学通报, 2023, 50(9): 3866-3880

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  • 收稿日期:2022-12-21
  • 最后修改日期:
  • 录用日期:2023-03-03
  • 在线发布日期: 2023-09-04
  • 出版日期: 2023-09-20
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