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2025年5月6日 周二
首页 > 过刊浏览>2023年第50卷第8期 >3588-3605. DOI:10.13344/j.microbiol.china.230168
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链霉菌启动子及其应用研究进展
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国家重点研发计划(2018YFA0903300)；国家自然科学基金(32071426)


Recent advances and applications of Streptomyces promoters
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  • 摘要
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  • 参考文献 [91]
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    摘要:

    链霉菌天然产物因其显著的生物活性一直是新药开发的重要来源，测序技术的发展揭示了链霉菌强大的生物合成潜力。链霉菌中多数次级代谢生物合成基因簇(biosynthetic gene clusters, BGCs)在常规实验条件下表达水平低甚至不表达，这使得相关天然产物的开发受到阻碍。原位激活和异源表达是挖掘链霉菌天然产物的有效方式，启动子作为基因表达的“开关”，在其中发挥着重要作用。因此对启动子的研究可以有效地促进BGCs的激活，从而挖掘新天然产物。本文重点阐述了链霉菌启动子的结构特征及其挖掘表征和设计构建的思路，并列举了链霉菌启动子在天然产物开发中的应用，有望为链霉菌生物合成路径的优化以及全新生物活性物质的发现提供思路和方法学参考。

    Abstract:

    Natural products with remarkable biological activities from Streptomyces are important sources for drug discovery. The advancing sequencing technology has revealed the biosynthetic potential of Streptomyces. Most biosynthetic gene clusters (BGCs) in Streptomyces are at low expression levels or even in silence under routine laboratory conditions, which hinders the discovery of natural products. In situ activation and heterologous expression are effective ways for discovering natural products in Streptomyces, in which promoters as the “switch” of gene expression play a key role. Therefore, the study of promoters can promote the activation of BGCs for the mining of new natural products. We introduce Streptomyces promoters in terms of the structures, mining, design, and applications in natural product discovery. This review is expected to provide new insights and methodological references for the optimization of biosynthesis pathways and the discovery of new bioactive substances in Streptomyces.

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董冀欣,周群,罗云孜. 链霉菌启动子及其应用研究进展[J]. 微生物学通报, 2023, 50(8): 3588-3605

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  • 收稿日期:2023-03-04
  • 录用日期:2023-04-07
  • 在线发布日期: 2023-08-08
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