马鞍菌属3个中国新记录

Three new records of Helvella in China

摘要:

【背景】目前国内学者已发表60个马鞍菌属物种，但山西省尚缺乏马鞍菌属真菌分布的系统性报道。【目的】调查我国山西省马鞍菌属的种类组成及地理分布。【方法】采集标本，对其进行详细的形态特征描述，并提取DNA，测定热激蛋白(heat shock protein, hsp)和核糖体大亚基(large subunit, LSU)序列，基于贝叶斯法(Bayesian inference, BI)和最大似然法(maximum likelihood, ML)构建分子系统发育树。【结果】鉴定结果表明这3个马鞍菌属物种分别为类帽状马鞍菌(Helvella capucinoides)、丹麦马鞍菌(H. danica)和柔毛马鞍菌(H. pubescens)。它们在形态上与模式标本的描述基本一致。基于hsp和LSU的系统发育关系分析也支持形态学的鉴定结果。【结论】参考我国截至目前已报道的马鞍菌属物种，确定这3个种均为中国新记录种。

Abstract:

[Background] Chinese scholars have reported 60 species of Helvella, while there is still a lack of systematic reports on the distribution of Helvella in Shanxi Province, China. [Objective] To investigate the species and geographical distribution of Helvella in Shanxi Province. [Methods] We collected Helvella species and detailed their morphology. After DNA extraction, we sequenced the heat shock protein (hsp) and ribosome large subunit (LSU) of each species and constructed phylogenetic trees based on Bayesian inference and maximum likelihood method. [Results] The three species were identified as H. capucinoides, H. danica, and H. pubescens, respectively. Their morphology was basically consistent with the description of the type specimens. The phylogenetic trees built based on hsp and LSU supported the identification results based on morphological characteristics. [Conclusion] In review of the Helvella species reported hitherto, we confirmed that these three species are new records in China.