两种盐生植物内生真菌多样性及其功能预测

Diversity and roles of endophytic fungi in two halophytes

摘要:

【背景】植物内生菌是微生物群落中一类非常重要的组成部分，是重要的微生物资源库，在植物促生、抗逆等多个领域有重要的研究和应用价值。【目的】进一步了解干旱荒漠区盐生植物内生真菌的多样性、群落结构和潜在功能特征。【方法】对生长在乌兹别克斯坦西咸海岸边的两种盐生植物毛足假木贼(Anabasis eriopoda abbreviated as AE)和展枝假木贼(A. truncata abbreviated as AT)的内生真菌群落进行扩增子测序分析。【结果】共获得166个amplicon sequencing variants (ASVs)，涉及4门49属，其中Neocamarosporium、Botryosphaeria和Alternaria及其所属高级分类单元是优势类群。多样性和群落组成分析显示两种盐生植物的内生真菌存在较为明显的差异，并包含一些潜在的新分类单元。基于PICRUSt2和FUNGuild的功能预测结果表明这两种盐生植物内生真菌的潜在功能和营养方式多样且表现出宿主差异性。【结论】盐生植物内生真菌具有较高的多样性和潜在的资源价值，有待进一步挖掘和研究。

Abstract:

[Background] Endophytes associated with halophytes have a wide range of activities, such as promoting plant growth, improving stress resistance, fixing nitrogen, and degrading toxic compounds. [Objective] To deeply understand the diversity, community structure, and potential roles of endophytic fungi of halophytes in the arid land. [Methods] Amplicon sequencing was performed for the endophytic fungi in two halophytes (Anabasis eriopoda and A. truncata) growing on the coast of the western Aral Sea in Uzbekistan. [Results] A total of 166 amplicon sequencing variants (ASVs) were obtained, representing 49 genera of 4 phyla, among which the dominant taxa were Neocamarosporium, Botryosphaeria, Alternaria and their higher taxa. The diversity and community composition of endophytic fungi showed significant differences between the two halophytes, which harbored potential novel taxa. Functional prediction by PICRUSt2 and FUNGuild demonstrated that the endophytic fungi in the two halophytes exhibited diverse roles and trophic modes, being host specific. [Conclusion] Our results suggest that the endophytic fungi in halophytes have high diversity and potential resource values and remain to be studied.