科微学术

微生物学通报

2025年4月22日 周二
首页 > 过刊浏览>2023年第50卷第7期 >3211-3231. DOI:10.13344/j.microbiol.china.220981
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食药用菌液体发酵及功能活性成分研究现状与展望
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上海市现代农业产业技术体系(沪农科产字[2022]第9号)；现代农业产业技术体系专项资金(CARS-20)；上海市农业科学院卓越团队建设计划(2022A-03)；上海市农业科学院攀高计划(2022-2024)


Liquid fermentation technology and functional components of edible and medicinal fungi
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  • 参考文献 [93]
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    摘要:

    利用食药用菌液体发酵技术可获得品质稳定、产物可控、富含多种有效营养和活性成分的功能性原料。本文系统总结了食药用菌液体发酵技术的研究现状和瓶颈，以及食药用菌液体发酵产物中的主要成分和功效，并与栽培子实体进行了比较。通过对食药用菌液体发酵技术瓶颈突破的展望，为食药用菌液体发酵产物的进一步应用提供参考。

    Abstract:

    The use of liquid fermentation technology can obtain functional raw materials from edible and medicinal fungi with stable quality, controllable products, and various active ingredients. This paper systematically summarized the research status and bottlenecks of liquid fermentation technology of edible and medicinal fungi, as well as the main components and efficacy of the liquid fermentation products of edible and medicinal fungi, and compared them with the cultivated fruiting bodies. Through the prospect of the breakthrough of liquid fermentation technology of edible and medicinal fungi, this paper is expected to provide references for the further application of liquid fermentation products of edible and medicinal fungi.

    参考文献
    [1] 游明乐. 中国食(药)用真菌发酵工程研究进展[J]. 微生物学通报, 2007, 34(2):327-331. YOU ML. The food Chinese (medicine) uses the fungus fermentation engineering research progress[J]. Microbiology China, 2007, 34(2):327-331 (in Chinese).
    [2] WU F, ZHOU LW, YANG ZL, BAU T, LI TH, DAI YC. Resource diversity of Chinese macrofungi:edible, medicinal and poisonous species[J]. Fungal Diversity, 2019, 98(1):1-76.
    [3] 魏滔, 张长生, 陈琼华, 周玉萍, 田长恩. 灵芝真菌液体发酵及其产物应用的研究进展[J]. 微生物学通报, 2022, 49(1):336-351. WEI T, ZHANG CS, CHEN QH, ZHOU YP, TIAN CE. Liquid fermentation of Ganoderma and application of its products[J]. Microbiology China, 2022, 49(1):336-351 (in Chinese).
    [4] 王谦, 胡卫静. 大型食药用真菌深层发酵研究进展[J]. 食品安全质量检测学报, 2016, 7(3):1240-1246. WANG Q, HU WJ. Research progress on submerged fermentation of macroscopic edible fungi[J]. Journal of Food Safety & Quality, 2016, 7(3):1240-1246 (in Chinese).
    [5] 邢燕, 杨晓彤, 杨庆尧. 灵芝液体深层发酵研究进展[J]. 工业微生物, 2007, 37(3):63-67. XING Y, YANG XT, YANG QY. Recent advances in liquid submerged fermentation for Ganoderma mushroom[J]. Industrial Microbiology, 2007, 37(3):63-67 (in Chinese).
    [6] 郭尚, 王慧娟. 食用菌深层发酵技术及其应用[J]. 山西农业科学, 2013, 41(8):885-888. GUO S, WANG HJ. Submerged fermentation technology of edible fungus and its application[J]. Journal of Shanxi Agricultural Sciences, 2013, 41(8):885-888 (in Chinese).
    [7] 杜德清, 王丽霞, 陈志生. 液体深层发酵技术在食用菌方面的应用[J]. 浙江林业科技, 2003, 23(3):83-85. DU DQ, WANG LX, CHEN ZS. Application of fermentation technique in edible mushroom cultivation[J]. Journal of Zhejiang Forestry Science and Technology, 2003, 23(3):83-85 (in Chinese).
    [8] HUMFELD H, SUGIHARA TF. Mushroom mycelium production by submerged propagation[J]. Food Technology, 1949, 3:353.
    [9] 上海市卫生局. 猴菇菌片[J]. 中草药通讯, 1978(1):29-30. Shanghai Health Bureau. Hougujun Pian[J]. Zhongcaoyao Tongxun, 1978(1):29-30 (in Chinese).
    [10] 胡焕荣. 灵芝菌丝体深层发酵工业化生产的研究[J]. 食品科学, 2006, 27(2):196-198. HU HR. Study on commercial production from deep submerged fermentation of Ganoderma lucidum mycelia[J]. Food Science, 2006, 27(2):196-198 (in Chinese).
    [11] FENG J, FENG N, TANG QJ, LIU YF, YANG Y, LIU F, ZHANG JS, LIN CC. Optimization of Ganoderma lucidum polysaccharides fermentation process for large-scale production[J]. Applied Biochemistry and Biotechnology, 2019, 189(3):972-986.
    [12] 李洁, 丁重阳, 顾正华, 张梁, 石贵阳. 混合碳源对灵芝多糖发酵及其抗肿瘤活性的影响[J]. 食品与生物技术学报, 2017, 36(2):129-135. LI J, DING ZY, GU ZH, ZHANG L, SHI GY. Effects of mixed carbon sources on production and antitumor activity of Ganoderma lucidum exopolysaccharides by submerged culture[J]. Journal of Food Science and Biotechnology, 2017, 36(2):129-135 (in Chinese).
    [13] 刘锡, 葛萃萃, 罗倩, 王莹, 向准, 刘忠玄, 杨澍雨. 红托竹荪液体菌种发酵条件配方优化研究[J]. 种子, 2021, 40(8):127-131. LIU X, GE CC, LUO Q, WANG Y, XIANG Z, LIU ZX, YANG SY. Study on optimization of fermentation conditions for liquid strains of Dictyophora rubrovolvata[J]. Seed, 2021, 40(8):127-131 (in Chinese).
    [14] 翟双星, 冯杰, 唐庆九, 冯娜, 杨焱, 刘艳芳, 唐传红, 周帅, 张劲松. 复合有机氮源对灵芝三萜液态深层发酵的影响[J]. 菌物学报, 2018, 37(12):1761-1770. ZHAI SX, FENG J, TANG QJ, FENG N, YANG Y, LIU YF, TANG CH, ZHOU S, ZHANG JS. Effects of complex organic nitrogen source on triterpene production by Ganoderma lingzhi based on liquid submerged fermentation[J]. Mycosystema, 2018, 37(12):1761-1770 (in Chinese).
    [15] LONG ZD, XUE Y, NING ZX, SUN JS, LI JG, SU Z, LIU QB, XU CP, YAN JK. Production, characterization, and bioactivities of exopolysaccharides from the submerged culture of Ganoderma cantharelloideum M. H. Liu[J]. 3 Biotech, 2021, 11(3):145.
    [16] 王小谭. 碳氮源对秀珍菇液体培养及胞外酶活性的影响[D]. 合肥:安徽农业大学硕士学位论文, 2019. WANG XT. Effects of carbon and nitrogen on liquid culture and extracellular enzyme activity of Pleurotus geesteranus[D]. Hefei:Master's Thesis of Anhui Agricultural University, 2019 (in Chinese).
    [17] 孟江玲, 何德, 李翠新. 蛹虫草对金属锌的耐受性与富集特征[J]. 北方园艺, 2021(6):123-128. MENG JL, HE D, LI CX. Tolerance and enrichment characteristics of Cordyceps militaris to metal zinc[J]. Northern Horticulture, 2021(6):123-128 (in Chinese).
    [18] 邓百万, 陈文强. 云芝液体培养及富集硒研究[J]. 氨基酸和生物资源, 2000, 22(4):21-24. DENG BW, CHEN WQ. Study on accumulation of selenium in Polystictus versicolor in submerged culture[J]. Amino Acids & Biotic Resources, 2000, 22(4):21-24 (in Chinese).
    [19] 叶盛权, 吴晖, 余以刚, 陈娜, 李金燕. 不同金属离子对灵芝多糖液态发酵的影响[J]. 食品研究与开发, 2011, 32(1):106-108. YE SQ, WU H, YU YG, CHEN N, LI JY. Research for different metal ion in Ganoderma lucidum polysaccharides fermentation[J]. Food Research and Development, 2011, 32(1):106-108 (in Chinese).
    [20] 赵小瑞, 贠建民, 艾对元, 张紊玮, 赵风琴, 李宏珍, 贾亚莉. 4种中草药提取物对灵芝液态发酵三萜产物形成的影响[J]. 食品与发酵工业, 2016, 42(3):97-103. ZHAO XR, YUN JM, AI DY, ZHANG WW, ZHAO FQ, LI HZ, JIA YL. Effects of four kinds of Chinese herbs extracts on Ganoderma triterpenoids production of Ganoderma japonicum in submerged fermentation[J]. Food and Fermentation Industries, 2016, 42(3):97-103 (in Chinese).
    [21] YANG XB, YANG YY, ZHANG YF, HE JH, XIE YZ. Enhanced exopolysaccharide production in submerged fermentation of Ganoderma lucidum by Tween 80 supplementation[J]. Bioprocess and Biosystems Engineering, 2021, 44(1):47-56.
    [22] 叶丽云, 林强, 刘梅, 吴小平. 钙离子和水杨酸诱导灵芝多糖和三萜的合成[J]. 菌物学报, 2017, 36(2):220-228. YE LY, LIN Q, LIU M, WU XP. The synthesis of polysaccharides and triterpenoids of Ganoderma lingzhi induced by calcium ion and salicylic acid[J]. Mycosystema, 2017, 36(2):220-228 (in Chinese).
    [23] 姚强, 高兴喜, 宫志远, 任鹏飞, 仇可新, 刘岩. 部分稀土元素对灵芝多糖和三萜类物质液体发酵的影响[J]. 食品科学, 2011, 32(5):224-227. YAO Q, GAO XX, GONG ZY, REN PF, QIU KX, LIU Y. Effects of some rare earth elements on liquid fermentation of Ganoderma lucidum to produce polysaccharides and triterpenoids[J]. Food Science, 2011, 32(5):224-227 (in Chinese).
    [24] 朱强, 夏艳秋, 汪志君, 杨从发. 4种中药对灵芝生长与发酵的影响[J]. 中国酿造, 2010, 29(7):163-165. ZHU Q, XIA YQ, WANG ZJ, YANG CF. Effects of four herbs on growth and fermentation of Ganoderma lucidum[J]. China Brewing, 2010, 29(7):163-165 (in Chinese).
    [25] 辛燕花, 梁彬, 王颖霞, 刘阳, 白晓静. 灵芝-银杏双向液体发酵条件优化及抗氧化的研究[J]. 菌物学报, 2017, 36(10):1427-1435. XIN YH, LIANG B, WANG YX, LIU Y, BAI XJ. Optimization of the Ganoderma lucidum-Ginkgo biloba bi-directional liquid fermentation condition and antioxidation properties of its products[J]. Mycosystema, 2017, 36(10):1427-1435 (in Chinese).
    [26] MA ZB, XU MM, WANG Q, WANG F, ZHENG HH, GU ZH, LI YR, SHI GY, DING ZY. Development of an efficient strategy to improve extracellular polysaccharide production of Ganoderma lucidum using l-phenylalanine as an enhancer[J]. Frontiers in Microbiology, 2019, 10:2306.
    [27] 裴海生, 孙君社, 王民敬, 尹腾, 张秀清. 木质素对灵芝菌丝体生长的影响[J]. 农业工程学报, 2017, 33(6):309-314. PEI HS, SUN JS, WANG MJ, YIN T, ZHANG XQ. Effect of lignin on growth of Ganoderma lucidum[J]. Transactions of the Chinese Society of Agricultural Engineering, 2017, 33(6):309-314 (in Chinese).
    [28] 李平作, 章克昌. 灵芝胞外多糖的分离纯化及生物活性[J]. 微生物学报, 2000, 40(2):217-220. LI PZ, ZHANG KC. Isolation, purification and bioactivities of exopoly saccharides from fermented broth of Ganoderma lucidum[J]. Acta Microbiologica Sinica, 2000, 40(2):217-220 (in Chinese).
    [29] 苏晓薇, 唐庆九, 张劲松, 冯娜, 王金艳, 周帅, 冯杰, 俞苓. 油酸促进灵芝三萜液态深层发酵的工艺研究及规模化验证[J]. 菌物学报, 2021, 40(9):2445-2460. SU XW, TANG QJ, ZHANG JS, FENG N, WANG JY, ZHOU S, FENG J, YU L. Synthesis of triterpenes in liquid submerged fermentation of Ganoderma lingzhi promoted by oleic acid[J]. Mycosystema, 2021, 40(9):2445-2460 (in Chinese).
    [30] 崔月花, 缪婧, 刘建凤, 陈清颖. 两阶段温度控制发酵对桑黄菌丝生长和胞外多糖产量的影响[J]. 食用菌学报, 2018, 25(4):57-64. CUI YH, MIAO J, LIU JF, CHEN QY. Effect of two-stage temperature control strategy on mycelial growth and exopolysaccharide production in submerged culture of Phellinus linteus[J]. Acta Edulis Fungi, 2018, 25(4):57-64 (in Chinese).
    [31] WANG CC. Enhanced exopolysaccharide production by Cordyceps militaris using repeated batch cultivation[J]. Journal of Bioscience and Bioengineering, 2019, 127(4):499-505.
    [32] 温鲁, 夏敏, 宋虎卫, 蒋洁, 袁丞墅. 液体培养蛹虫草虫草素和腺苷的代谢量[J]. 微生物学通报, 2005, 32(3):91-94. WEN L, XIA M, SONG HW, JIANG J, YUAN CS. The metabolism yield of cordycepin and adenosine in Cordyceps militaris by liquid culture[J]. Microbiology, 2005, 32(3):91-94 (in Chinese).
    [33] 刘艳芳, 唐庆九, 顾俊杰, 张劲松, 杨焱, 冯娜, 周帅, 魏东芝. 北冬虫夏草深层发酵高产虫草素工艺的优化[J]. 上海农业学报, 2010, 26(3):26-30. LIU YF, TANG QJ, GU JJ, ZHANG JS, YANG Y, FENG N, ZHOU S, WEI DZ. Optimization of submerged fermentation process of Cordyceps militaris to produce a high cordycepin content[J]. Acta Agriculturae Shanghai, 2010, 26(3):26-30 (in Chinese).
    [34] 王焱, 连洁, 王竟夷, 廖晓晓, 班立桐, 王玉. 真姬菇液态发酵关键影响因素的优化[J]. 园艺与种苗, 2021, 41(10):27-29, 33. WANG Y, LIAN J, WANG JY, LIAO XX, BAN LT, WANG Y. Optimization of key factors affecting the liquid fermentation of Hypsizygus marmoreus[J]. Horticulture & Seed, 2021, 41(10):27-29, 33 (in Chinese).
    [35] 裘娟萍, 孙培龙, 朱家荣, 钟卫鸿. 灰树花深层发工艺条件的研究[J]. 微生物学通报, 2001, 28(3):33-35. QIU JP, SUN PL, ZHU JR, ZHONG WH. Studies of the technological conditions for submerged culture of Grifola frondosa[J]. Microbiology China, 2001, 28(3):33-35 (in Chinese).
    [36] 谭周进, 谢达平, 王征, 李立恒. 蜜环菌胞外多糖的发酵条件研究[J]. 微生物学通报, 2002, 29(3):33-37. TAN ZJ, XIE DP, WANG Z, LI LH. Studies on the production condition of exopolysaccharide from Armillarla mellea[J]. Microbiology China, 2002, 29(3):33-37 (in Chinese).
    [37] 吴晓彤. 蒙古口蘑分子鉴定及其菌丝体液体发酵技术与应用[D]. 呼和浩特:内蒙古大学博士学位论文, 2014. WU XT. Molecular identification and liquid fermentation of Tricholoma mongolicum and application of mycelium metabolite[D]. Hohhot:Doctoral Dissertation of Inner Mongolia University, 2014 (in Chinese).
    [38] 刘萍, 陶文沂, 许正宏, 敖宗华, 孙志浩, 尹光耀. 松口蘑深层发酵工艺的研究[J]. 微生物学通报, 2002, 29(5):5-9. LIU P, TAO WY, XU ZH, AO ZH, SUN ZH, YIN GY. Deep fermentation technique of Tricholoma matsutake mycelium[J]. Microbiology China, 2002, 29(5):5-9 (in Chinese).
    [39] 冀颐之, 杜连祥. 深层培养裂褶菌胞外多糖的提取及结构研究[J]. 微生物学通报, 2003, 30(5):15-20. JI YZ, DU LX. Extraction and structural study of schizophyllan from Schizophyllum commune by submerged cultivation[J]. Microbiology China, 2003, 30(5):15-20 (in Chinese).
    [40] 卢征华, 文庭池, 徐彦军, 余刚江, 杨雨. 红托竹荪液体菌种培养基及培养条件优化[J]. 北方园艺, 2021(22):124-129. LU ZH, WEN TC, XU YJ, YU GJ, YANG Y. Optimization for the medium for liquid spawn and culture conditions of Dictyophora rubrovolvata[J]. Northern Horticulture, 2021(22):124-129 (in Chinese).
    [41] 蔺银鼎, 马艳弘, 张福元, 牛颜冰. 竹荪液体培养基优化配方筛选及培养基营养变化特性的研究[J]. 食用菌学报, 2000, 7(4):18-24. LIN YD, MA YH, ZHANG FY, NIU YB. Study on the screening of optimal liquid medium formula and the characteristics of nutritional change in Dictyophora indusiate liquid medium[J]. Acta Edulis Fungi, 2000, 7(4):18-24 (in Chinese).
    [42] 冯杰, 冯娜, 刘艳芳, 唐庆九, 周帅, 刘方, 张劲松, 杨焱. 面向规模化应用的竹荪多糖液态深层发酵工艺优化[J]. 食品科学, 2020, 41(2):181-187. FENG J, FENG N, LIU YF, TANG QJ, ZHOU S, LIU F, ZHANG JS, YANG Y. Optimization of medium components for large-scale production of intracellular polysaccharides from Dictyophora indusiata in submerged fermentation[J]. Food Science, 2020, 41(2):181-187 (in Chinese).
    [43] 颜梦秋, 田振, 冯娜, 冯杰, 张劲松. 棘托竹荪发酵物的体外抗肿瘤活性[J]. 食用菌学报, 2016, 23(2):75-78. YAN MQ, TIAN Z, FENG N, FENG J, ZHANG JS. Inhibition of in vitro proliferation of selected cancer cell lines by extracts of Dictyophora echinovolvata mycelium and spent culture medium[J]. Acta Edulis Fungi, 2016, 23(2):75-78 (in Chinese).
    [44] 王云龙. 羊肚菌液体发酵培养条件优化[D]. 大连:大连工业大学硕士学位论文, 2014. WANG YL. The optimization of liquid fermentation conditions of Morchella[D]. Dalian:Master's Thesis of Dalian Polytechnic University, 2014 (in Chinese).
    [45] 于湘丽. 松茸菌液体发酵及多糖抗肿瘤活性的研究[D]. 长春:吉林大学硕士学位论文, 2014. YU XL. Study of the liquid fermentation of Tricholoma matsutake and the anti-tumor activity of polysaccharides[D]. Changchun:Master's Thesis of Jilin University, 2014 (in Chinese).
    [46] 李作美. 鼎湖鳞伞液态发酵和菌丝体多糖的提取、纯化及生物活性的研究[D]. 南京:南京农业大学硕士学位论文, 2012. LI ZM. The research on submerged fermentation vs extracted and purified of polysaccharide and biological activity of Pholiota dinghuensis BI[D]. Nanjing:Master's Thesis of Nanjing Agricultural University, 2012 (in Chinese).
    [47] FENG J, FENG N, ZHANG JS, YANG Y, JIA W, LIN CC. A new temperature control shifting strategy for enhanced triterpene production by Ganoderma lucidum G0119 based on submerged liquid fermentation[J]. Applied Biochemistry and Biotechnology, 2016, 180(4):740-752.
    [48] 乔双逵, 彭林, 丁重阳, 顾正华, 张梁, 石贵阳. 液体发酵条件对灵芝菌体形态及胞外多糖活性的影响[J]. 食品与生物技术学报, 2014, 33(10):1070-1076. QIAO SK, PENG L, DING ZY, GU ZH, ZHANG L, SHI GY. Effect of different culture conditions on mycelium morphology and activity of exopolysaccharides from Ganoderma lucidum in submerged culture[J]. Journal of Food Science and Biotechnology, 2014, 33(10):1070-1076 (in Chinese).
    [49] 潘江安, 唐庆九, 冯娜, 唐传红, 王金艳, 刘艳芳, 袁峰, 徐国华, 颜梦秋, 谭贻, 张劲松, 周帅, 冯杰. 利用阶段pH控制提高灵芝深层发酵合成三萜能力的研究[J]. 食用菌学报, 2020, 27(2):84-91. PAN JA, TANG QJ, FENG N, TANG CH, WANG JY, LIU YF, YUAN F, XU GH, YAN MQ, TAN Y, ZHANG JS, ZHOU S, FENG J. Enhanced triterpene biosynthesis in Ganoderma lucidum by staged pH control during submerged fermentation[J]. Acta Edulis Fungi, 2020, 27(2):84-91 (in Chinese).
    [50] FENG J, FENG N, YANG Y, LIU F, ZHANG JS, JIA W, LIN CC. Simple and reproducible two-stage agitation speed control strategy for enhanced triterpene production by Lingzhi or reishi medicinal mushrooms, Ganoderma lucidum ACCC G0119 (higher basidiomycetes) based on submerged liquid fermentation[J]. International Journal of Medicinal Mushrooms, 2015, 17(12):1151-1159.
    [51] 冯杰, 冯娜, 杨焱, 刘方, 贾薇, 张劲松. 通气量对灵芝菌丝体液态深层发酵合成灵芝三萜的影响[J]. 天然产物研究与开发, 2015, 27(9):1564-1570. FENG J, FENG N, YANG Y, LIU F, JIA W, ZHANG JS. Effects of aeration rate on the production of triterpenes by Ganoderma lucidum G0119 in liquid submerged fermentation[J]. Natural Product Research and Development, 2015, 27(9):1564-1570 (in Chinese).
    [52] 吴迪, 袁峰, 王国瑞, 周帅, 杨焱, 冯娜, 唐庆九, 刘艳芳, 朱增亮, 徐国华, 唐传红, 刘方, 张劲松, 冯杰. 基于通气量调控的灵芝菌丝体胞内多糖发酵工艺优化[J]. 食用菌学报, 2019, 26(1):35-40. WU D, YUAN F, WANG GR, ZHOU S, YANG Y, FENG N, TANG QJ, LIU YF, ZHU ZL, XU GH, TANG CH, LIU F, ZHANG JS, FENG J. A aeration volume strategy for enhanced intracellular polysaccharide production by Ganoderma lucidum mycelium based on submerged culture[J]. Acta Edulis Fungi, 2019, 26(1):35-40 (in Chinese).
    [53] 冯杰, 冯娜, 唐庆九, 颜梦秋, 杨焱, 周帅, 刘艳芳, 刘方, 张劲松. 补料方式对灵芝菌丝体液态深层发酵合成灵芝三萜的影响[J]. 食品科学, 2017, 38(12):57-62. FENG J, FENG N, TANG QJ, YAN MQ, YANG Y, ZHOU S, LIU YF, LIU F, ZHANG JS. Effects of different feeding methods on production of triterpenes by Ganoderma lucidum in submerged fermentation[J]. Food Science, 2017, 38(12):57-62 (in Chinese).
    [54] WEI ZH, LIU LL, GUO XF, LI YJ, HOU BC, FAN QL, WANG KX, LUO YD, ZHONG JJ. Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26[J]. Bioprocess and Biosystems Engineering, 2016, 39(1):37-44.
    [55] TANG YJ, ZHANG W, LIU RS, ZHU LW, ZHONG JJ. Scale-up study on the fed-batch fermentation of Ganoderma lucidum for the hyperproduction of ganoderic acid and Ganoderma polysaccharides[J]. Process Biochemistry, 2011, 46(1):404-408.
    [56] 尹川, 杜冰冰, 连洁, 张弘扬, 黄亮, 班立桐, 王玉. 气升式反应器中通气量和pH对双孢蘑菇液体菌种的影响[J]. 食品与发酵科技, 2021, 57(2):35-39. YIN C, DU BB, LIAN J, ZHANG HY, HUANG L, BAN LT, WANG Y. Effects of aeration volume and pH on liquid seeds of Agaricus bisporus in 5 L air-lift reactor[J]. Food and Fermentation Sciences & Technology, 2021, 57(2):35-39 (in Chinese).
    [57] 赵炳杰, 郭岩彬. 食用菌多糖的提取纯化及生物活性研究进展[J]. 中国生物工程杂志, 2022, 42(S1):146-159. ZHAO BJ, GUO YB. Advances in extraction, purification and bioactivity of polysaccharides from edible fungi[J]. China Biotechnology, 2022, 42(S1):146-159 (in Chinese).
    [58] JING XY, MAO DB, GENG LJ, XU CP. Medium optimization, molecular characterization, and bioactivity of exopolysaccharides from Pleurotus eryngii[J]. Archives of Microbiology, 2013, 195(10):749-757.
    [59] ASSIS IS, CHAVES MB, SILVEIRA MLL, GERN RMM, WISBECK E, JÚNIOR AF, FURLAN SA. Production of bioactive compounds with antitumor activity against sarcoma 180 by Pleurotus sajor-caju[J]. Journal of Medicinal Food, 2013, 16(11):1004-1012.
    [60] 罗钦, 徐军伟. 液体发酵灵芝胞外多糖和胞内多糖的单糖组成及抗氧化活性[J]. 食用菌学报, 2021, 28(6):135-142. LUO Q, XU JW. Monosaccharide composition and antioxidant activity of extracellular polysaccharides and intracellular polysaccharides in liquid ferment of Ganoderma lucidum[J]. Acta Edulis Fungi, 2021, 28(6):135-142 (in Chinese).
    [61] DU B, YANG YD, BIAN ZX, XU BJ. Characterization and anti-inflammatory potential of an exopolysaccharide from submerged mycelial culture of Schizophyllum commune[J]. Frontiers in Pharmacology, 2017, 8:252.
    [62] 徐慧, 陈蕾蕾, 赵双芝, 裘纪莹, 孙华, 辛雪. 灵芝菌丝体的三萜类化合物及其抗肿瘤活性研究进展[J]. 齐鲁工业大学学报(自然科学版), 2016, 30(4):26-29. XU H, CHEN LL, ZHAO SZ, QIU JY, SUN H, XIN X. Recent progress in anti-tumor activities of Ganoderma triterpene from the mycelia of liquid-cultured Ganoderma lucidum[J]. Journal of Qilu University of Technology (Natural Science Edition), 2016, 30(4):26-29 (in Chinese).
    [63] 朱晓璐, 岳亚文, 张劲松, 冯杰, 唐庆九, 冯娜, 韩伟. 灵芝菌丝体中一个三萜类化合物的核磁归属及活性初探[J]. 菌物学报, 2020, 39(8):1551-1558. ZHU XL, YUE YW, ZHANG JS, FENG J, TANG QJ, FENG N, HAN W. NMR attribution and bioactivity evaluation of a triterpene in mycelia of Ganoderma lingzhi[J]. Mycosystema, 2020, 39(8):1551-1558 (in Chinese).
    [64] 蔡程山. 桑黄液体发酵菌丝体总三萜的提取及活性分析[D]. 北京:北京林业大学硕士学位论文, 2018. CAI CS. Extraction and activity analysis of total triterpenoids from mycelium of Sanghuangporus sanghuang liquid fermentation[D]. Beijing:Master's Thesis of Beijing Forestry University, 2018 (in Chinese).
    [65] 周旭, 庄思远, 李彦杰, 刘箬瑶, 刘春明, 李赛男, 张语迟. 赤灵芝中三萜类活性成分的提取筛选和质谱分析[J]. 现代食品科技, 2022, 38(6):169-178. ZHOU X, ZHUANG SY, LI YJ, LIU RY, LIU CM, LI SN, ZHANG YC. Extraction, screening and mass spectrometry analysis of active triterpenoid components in Ganoderma lucidum[J]. Modern Food Science and Technology, 2022, 38(6):169-178 (in Chinese).
    [66] AHLBORN J, STEPHAN A, MECKEL T, MAHESHWARI G, RÜHL M, ZORN H. Upcycling of food industry side streams by basidiomycetes for production of a vegan protein source[J]. International Journal of Recycling of Organic Waste in Agriculture, 2019, 8(1):447-455.
    [67] KURBANOGLU EB. Submerged production of edible mushroom Agaricus bisporus mycelium in ram horn hydrolysate[J]. Industrial Crops and Products, 2004, 19(3):225-230.
    [68] RAHGO Z, SAMADLOUIE HR, MOJERLOU S, JAHANBIN K. Statistical optimization of culture conditions for protein production by a newly isolated Morchella fluvialis[J]. BioMed Research International, 2019, 2019:7326590.
    [69] GANG J, LIU H, LIU YH. Optimization of liquid fermentation conditions and protein nutrition evaluation of mycelium from the caterpillar medicinal mushroom, Cordyceps militaris (ascomycetes)[J]. International Journal of Medicinal Mushrooms, 2016, 18(8):745-752.
    [70] BISHOP KS, KAO CHJ, XU YY, GLUCINA MP, PATERSON RRM, FERGUSON LR. From 2000years of Ganoderma lucidum to recent developments in nutraceuticals[J]. Phytochemistry, 2015, 114:56-65.
    [71] BABY S, JOHNSON AJ, GOVINDAN B. Secondary metabolites from Ganoderma[J]. Phytochemistry, 2015, 114:66-101.
    [72] 刘艳芳, 唐庆九, 王金艳, 李传华, 冯娜, 王晨光, 唐传红, 张劲松. 白肉灵芝子实体和菌丝体活性成分的比较[J]. 食用菌学报, 2021, 28(4):20-26. LIU YF, TANG QJ, WANG JY, LI CH, FENG N, WANG CG, TANG CH, ZHANG JS. Comparison of bioactive components in fruiting body and fermented mycelium of Ganoderma leucocontextum[J]. Acta Edulis Fungi, 2021, 28(4):20-26 (in Chinese).
    [73] 张李阳, 熊晓辉, 沈昌, 沈爱光. 灵芝子实体及其深层培养产物特性的研究[J]. 中国食用菌, 1998, 17(1):15-17. ZHANG LY, XIONG XH, SHEN C, SHEN AG. Comparative study on products of fruiting body and submerged fermentation of Ganoderma sp[J]. Edible Fungi of China, 1998, 17(1):15-17 (in Chinese).
    [74] 席亚丽, 茆爱丽, 王晓琴, 桂建华, 王治江, 魏生龙. 荷叶离褶伞子实体、菌丝体及发酵液蛋白质营养价值评价[J]. 菌物学报, 2010, 29(4):603-607. XI YL, MAO AL, WANG XQ, GUI JH, WANG ZJ, WEI SL. Assessment for protein nutrition of fruit bodies, mycelia and fermentation broth of Lyophyllum decastes[J]. Mycosystema, 2010, 29(4):603-607 (in Chinese).
    [75] 席亚丽, 王治江, 王晓琴, 魏生龙. 荷叶离褶伞子实体、菌丝体和发酵液营养成分比较分析[J]. 食品科学, 2010, 31(6):155-157. XI YL, WANG ZJ, WANG XQ, WEI SL. Comparative analysis of nutrients in fruit body, mycelia and fermentation broth of Lyophyllum decastes[J]. Food Science, 2010, 31(6):155-157 (in Chinese).
    [76] 周选围, 林娟, 周良. 灵芝主要营养成分的测定分析[J]. 陕西师范大学学报(自然科学版), 1998, 26(S1):219-222. ZHOU XW, LIN J, ZHOU L. Determine and analysis on primary nutritional components of Ganoderma spp.[J]. Journal of Shaanxi Normal University (Natural Science Edition), 1998, 26(S1):219-222 (in Chinese)
    [77] 孙培龙, 宣以巍, 裘娟萍. 灰树花菌丝体发酵及成分分析[J]. 食用菌, 2001, 23(5):4-5. SUN PL, XUAN YW, QIU JP. Fermentation and composition analysis of Grifola frondosa mycelia[J]. Edible Fungi, 2001, 23(5):4-5 (in Chinese).
    [78] 马艳弘, 张福元. 竹荪子实体与菌丝体营养成分的测定分析[J]. 山西农业大学学报(自然科学版), 2004, 24(4):389-391. MA YH, ZHANG FY. Determination of the nutritive components of mycelia and fruitbody of Dictyophora indusiata[J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2004, 24(4):389-391 (in Chinese).
    [79] 高虹, 谷文英, 丁霄霖. 巴西蘑菇发酵菌丝体醇提物的抑瘤活性[J]. 食品与生物技术学报, 2006, 25(6):54-57. GAO H, GU WY, DING XL. Studies on antitumor activity of ethanol extracts from liquid-cultured mycelium of Agaricus brasiliensis sp[J]. Journal of Food Science and Biotechnology, 2006, 25(6):54-57 (in Chinese).
    [80] 黄静, 李爱欣, 周贤, 王淑敏. 松杉灵芝发酵产物对H22肝腹水瘤小鼠免疫功能影响及抗肿瘤作用[J]. 药学研究, 2016, 35(10):575-578. HUANG J, LI AX, ZHOU X, WANG SM. The immune function and anti-tumor effects of Ganoderma tsugae fermentation product on H22 liver ascites tumor mice[J]. Journal of Pharmaceutical Research, 2016, 35(10):575-578 (in Chinese).
    [81] FIJAŁKOWSKA A, MUSZYŃSKA B, SUŁKOWSKA-ZIAJA K, KAŁA K, PAWLIK A, STEFANIUK D, MATUSZEWSKA A, PISKA K, PĘKALA E, KACZMARCZYK P, PIĘTKA J, JASZEK M. Medicinal potential of mycelium and fruiting bodies of an arboreal mushroom Fomitopsis officinalis in therapy of lifestyle diseases[J]. Scientific Reports, 2020, 10:20081.
    [82] 李兆兰, 李学信. 裂褶菌胞内多糖的分离纯化鉴定及其性质[J]. 真菌学报, 1994, 13(4):267-272. LI ZL, LI XX. Isolation, purification and identification of schizophyllan[J]. Mycosystema, 1994, 13(4):267-272 (in Chinese).
    [83] CARRIERI R, MANCO R, SAPIO D, IANNACCONE M, FULGIONE A, PAPAIANNI M, de FALCO B, GRAUSO L, TARANTINO P, IANNIELLO F, LANZOTTI V, LAHOZ E, CAPPARELLI R. Structural data and immunomodulatory properties of a water-soluble heteroglycan extracted from the mycelium of an Italian isolate of Ganoderma lucidum[J]. Natural Product Research, 2017, 31(18):2119-2125.
    [84] 胡文继. 猴头菌发酵菌丝体纯化多糖的抗阿尔茨海默症活性研究[D]. 长春:吉林大学博士学位论文, 2021. HU WJ. Study on the anti-alzheimer's disease activity of purified polysaccharides from Hericium erinaceus fermentation mycelium[D]. Changchun:Doctoral Dissertation of Jilin University, 2021 (in Chinese).
    [85] 刘雨婷. 液体培养条件对桦褐孔菌产活性多糖的影响研究[D]. 长春:东北师范大学硕士学位论文, 2021. LIU YT. Study on the effect of different liquid fermentation conditions on the active polysaccharides from Inonotus obliquus[D]. Changchun:Master's Thesis of Northeast Normal University, 2021 (in Chinese).
    [86] 张强, 吴彩娥. 羊肚菌菌丝体蛋白的理化特性及抗氧化活性[J]. 浙江农业学报, 2016, 28(8):1408-1415. ZHANG Q, WU CE. Physicochemical properties and antioxidant activities of protein from Morchella esculenta mycelium[J]. Acta Agriculturae Zhejiangensis, 2016, 28(8):1408-1415 (in Chinese).
    [87] 窦会娟, 孙连海, 郭文涛. 食用菌发酵液对耐药菌的抑菌活性研究[J]. 中国酿造, 2015, 34(7):40-42. DOU HJ, SUN LH, GUO WT. Antibacterial activity of mushroom fermentation broth to drug-resistant bacteria[J]. China Brewing, 2015, 34(7):40-42 (in Chinese).
    [88] 于华峥, 刘艳芳, 周帅, 颜梦秋, 薛令坤, 唐庆九, 张劲松. 灵芝子实体、菌丝体及孢子粉中多糖成分差异比较研究[J]. 菌物学报, 2016, 35(2):170-177. YU HZ, LIU YF, ZHOU S, YAN MQ, XUE LK, TANG QJ, ZHANG JS. Comparison of the polysaccharides from fruiting bodies, mycelia and spore powder of Ganoderma lingzhi[J]. Mycosystema, 2016, 35(2):170-177 (in Chinese).
    [89] 蔡梦婷, 唐庆九, 王益莉, 黄怡雯, 冯杰, 冯娜, 俞苓. 灵芝孢子、子实体和菌丝体的抗氧化活性比较[J]. 食用菌学报, 2018, 25(3):72-76. CAI MT, TANG QJ, WANG YL, HUANG YW, FENG J, FENG N, YU L. Comparison on antioxidant activity of spore, fruiting Body and mycelium of Ganoderma lucidum[J]. Acta Edulis Fungi, 2018, 25(3):72-76 (in Chinese).
    [90] 张俊峰, 张忠, 汪雯翰, 张赫男, 冯娜, 吴迪, 刘艳芳, 杨焱. 桑黄菌丝体和子实体中次级代谢产物及其活性的比较[J]. 菌物学报, 2020, 39(2):398-408. ZHANG JF, ZHANG Z, WANG WH, ZHANG HN, FENG N, WU D, LIU YF, YANG Y. Comparison of active secondary metabolites between mycelia and fruiting bodies of Sanghuangporous sanghuang[J]. Mycosystema, 2020, 39(2):398-408 (in Chinese).
    [91] 陈艳芳, 鲍大鹏, 陈洪雨, 冯杰, 罗颖, 张茜, 邹根, 赵勇. 液体深层发酵刺芹侧耳菌丝体蛋白提取及其功能特性[J]. 食用菌学报, 2021, 28(3):112-121. CHEN YF, BAO DP, CHEN HY, FENG J, LUO Y, ZHANG X, ZOU G, ZHAO Y. Extraction and functional properties of proteins in Pleurotus eryngii mycelium from submerged liquid fermentation[J]. Acta Edulis Fungi, 2021, 28(3):112-121 (in Chinese).
    [92] 李艳丽, 金周雨, 李玉. 原生质体紫外诱变筛选刺芹侧耳高EPS含量菌株及其鉴定[J]. 中国农机化学报, 2016, 37(12):98-102. LI YL, JIN ZY, LI Y. Screening and identifying strains with high EPS content of Pleurotus eryngii by UV induced protoplast mutagenesis[J]. Journal of Chinese Agricultural Mechanization, 2016, 37(12):98-102 (in Chinese).
    [93] 杨珊, 杨焱, 李巧珍, 吴迪, 杨瑞恒, 汪文翰, 张赫男. 常压室温等离子体诱变筛选高产多糖猴头菌株的研究[J]. 上海农业学报, 2019, 35(5):6-11. YANG S, YANG Y, LI QZ, WU D, YANG RH, WANG WH, ZHANG HN. Screening of high-yield polysaccharide Hericium erinareus by atmospheric and room temperature plasma mutagenesis[J]. Acta Agriculturae Shanghai, 2019, 35(5):6-11 (in Chinese).
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颜梦秋,刘艳芳,周帅,唐传红,冯杰,张劲松. 食药用菌液体发酵及功能活性成分研究现状与展望[J]. 微生物学通报, 2023, 50(7): 3211-3231

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