芽孢杆菌DU-106裂解物对2,4-二硝基氟苯诱导的特应性皮炎小鼠的治疗作用

Therapeutic effect of Bacillus sp. DU-106 lysate on 2,4-dinitrofluorobenzene-induced atopic dermatitis in mice

摘要:

【背景】本团队前期研究发现芽孢杆菌DU-106具有良好的健康益处，但都是基于活菌的研究，对灭活菌的研究尚未开展。【目的】探究芽孢杆菌DU-106裂解物对特应性皮炎的治疗效果及作用机制。【方法】以2,4-二硝基氟苯诱导的特应性皮炎BALB/c小鼠为模型，通过病理学观察、免疫组化等手段研究其治疗效果，并测定NF-κB通路中基因与蛋白表达量，揭示其内在机制。【结果】芽孢杆菌DU-106裂解物有效缓解了特应性皮炎小鼠的病理学特征，减轻了肥大细胞的浸润，降低了免疫球蛋白E、肿瘤坏死因子-α (TNF-α)和干扰素-γ的含量(P<0.05)。芽孢杆菌DU-106裂解物可能是通过抑制NF-κB信号通路中TNF-α、IKKα和NF-κB基因表达水平和蛋白磷酸化而实现抗特应性皮炎效果。【结论】芽孢杆菌DU-106裂解物可作为一种后生元，对特应性皮炎具有积极的治疗作用。

Abstract:

[Background] Our research team have discovered that Bacillus sp. DU-106 has good health benefits in previous studies. However, the conclusion is based on the studies of living bacteria and the studies of inactivated bacteria remain to be carried out. [Objective] To investigate the effect and mechanism of Bacillus sp. DU-106 lysate in the treatment of atopic dermatitis. [Methods] Pathological observation and immunohistochemistry were employed to study the therapeutic effect of Bacillus sp. DU-106 lysate on the mouse model of 2,4-dinitrofluorobenzene-induced atopic dermatitis. The expression levels of the proteins involved in the nuclear factor kappa-B (NF-κB) signaling pathway were determined to reveal the mechanism. [Results] Bacillus sp. DU-106 lysate alleviated the pathological changes, reduced the infiltration of mast cells, and lowered the levels of immunoglobulin E, tumor necrosis factor-α (TNF-α), and interferon-γ (P<0.05) in the model mice. Furthermore, Bacillus sp. DU-106 lysate down-regulated the expression levels and inhibited the phosphorylation of TNF-α, inhibitory kappa B kinase α (IKKα), and NF-κB in the NF-κB signaling pathway in the model mice. [Conclusion] Bacillus sp. DU-106 lysate can be used as a probiotic with therapeutic effects on atopic dermatitis.