AA10家族裂解多糖单加氧酶的模块化组成及底物选择性的研究进展

Research progress in modular composition and substrate selectivity of AA10 lytic polysaccharide monooxygenases

摘要:

AA10家族裂解多糖单加氧酶(lytic polysaccharide monooxygenases, LPMOs)主要分布于细菌中，因其具有催化纤维素和几丁质等结晶多糖氧化降解的特性，在工业生物质转化过程中具有极强的应用潜力，从而受到广泛关注。然而，AA10家族不同LPMOs作用的底物种类及氧化位点和氧化产物也不尽相同，LPMOs的结构与组成对其底物选择性的影响机制有待进一步探究。因此，本文综述了AA10家族LPMOs的模块化结构组成及其催化机制，梳理了AA10家族LPMOs的底物谱，系统总结了AA10家族LPMOs的结构、关键作用残基及多模块组合对底物选择性影响的最新进展，并展望了LPMOs在生物质转化和生物燃料工业中广阔的应用前景。

Abstract:

AA10 lytic polysaccharide monooxygenases (LPMOs) mainly exist in bacteria. They have great application potential in industrial biomass conversion because of the ability in degrading crystalline polysaccharides such as cellulose and chitin and thus have attracted wide attention. However, the substrates, oxidation sites, and oxidation products vary among different AA10 LPMOs, and the influence mechanism of the structure and composition of LPMOs on the substrate selectivity remains to be explored. This paper introduced the modular structure, catalytic mechanism, and substrate spectrum of AA10 LPMOs. Further, we reviewed the research progress in the effects of structure, critical functional residues, and module combination of AA10 LPMOs on substrate selectivity. On this basis, we put forward the application prospects of LPMOs in biomass conversion and biofuel industry.