首发抑郁症患者肠道菌群多样性与抑郁症状的相关性分析

Correlation analysis of intestinal flora diversity and clinical symptoms in patients with first-episode depression

摘要:

【背景】脑-肠轴理论提示着肠道菌群和抑郁症之间存在着潜在的联系，但目前的研究争议较多，具体联系尚无定论。【目的】研究首发抑郁症患者肠道菌群多样性与抑郁症状的相关性，分析肠道菌群多样性及脱硫弧菌等菌群与抑郁症之间的潜在关系，为后续的纵向研究提供一定的理论支持。【方法】对抑郁组(n=23)和对照组(n=31)粪便中16S rRNA基因V4-V5区域片段进行测序，使用汉密尔顿抑郁量表对两组研究对象进行评估。使用α多样性检验、β多样性检验、t检验、皮尔逊相关性检验及斯皮尔曼相关性检验进行统计分析。【结果】抑郁组与对照组的肠道菌群多样性无明显差异(P>0.05)。两组肠道菌群在结构组成上有差异，属水平和种水平上，分别有28个菌属和40个菌种的相对丰度呈显著性差异(p<0.05)。抑郁组肠道菌群多样性(shannon、Chao1指数)与抑郁症状间存在显著的正相关(p<0.05)。属水平和种水平上菌群相对丰度和抑郁症状的相关性检验中，脱硫弧菌等与抑郁症状存在显著相关性(p<0.05)。【结论】首发抑郁症患者与健康人群肠道菌群在菌群结构组成上存在差异。抑郁组肠道菌群多样性与抑郁症状存在显著的相关性，其中脱硫弧菌等菌群相对丰度和抑郁症状间存在明显的正相关。

Abstract:

[Background] The brain-gut axis theory suggests that there is a potential link between intestinal flora and depression, but the current research is controversial, and the specific link is not yet determined. [Objective] To study the correlation between intestinal flora diversity and depressive symptoms in patients with first-episode depression, and to analyze the potential relationship between intestinal flora diversity and Desulfovibrio and depression, thereby providing theoretical support for subsequent longitudinal studies. [Methods] The V4-V5 region fragments in the 16S rRNA gene from the feces of the depression group (n=23) and the normal group (n=31) were genetically sequenced, and the Hamilton Depression Scale was used to evaluate the two groups. The α diversity test, β diversity test, t-test, Pearson correlation test, and Spearman correlation test were used for statistical analysis. [Results] There was no significant difference in the diversity of intestinal flora between the depression group and the control group (P>0.05). There were differences in the structure of intestinal flora between the two groups. The relative abundances of 28 genera and 40 species were significantly different at genus and species levels (P<0.05). There was a significant positive correlation between intestinal flora diversity (Shannon index and Chao1 index) and depressive symptoms (P<0.05). In the correlation test of the relative abundances and depressive symptoms at genus and species levels, Desulfovibrio was significantly correlated with depressive symptoms (P<0.05). [Conclusion] In this study, there are differences existed in the structure and composition of intestinal microbiota between patients with first-episode depression and healthy people. There is a significant correlation between intestinal flora diversity and depressive symptoms, and there is a significant positive correlation between the relative abundances of some flora such as Desulfovibrio and the depressive symptoms.