【背景】近年来，苯胺类化合物加重了生态环境的污染，而生物法处理苯胺类废水具有较大发展潜力与广阔的应用前景。【目的】从长期受苯胺类化合物污染的活性污泥中分离获得一株能高效降解苯胺的菌株，优化其培养基及降解条件，为苯胺生物修复提供菌株与基因资源。【方法】采用平板法从富集驯化的菌株中筛选出以苯胺为唯一碳氮源和能源的高效降解菌，通过16S rRNA基因测序鉴定菌种，利用单因素筛选实验对降解条件进行优化，通过正交试验优化培养条件。 【结果】筛选到一株苯胺降解菌BA-6，经鉴定为微杆菌属(Microbacterium)。菌株BA-6对初始浓度为600 mg/L苯胺的日降解率可达98%以上。其高效降解的温度范围是30-37 ℃，pH范围是6.5-7.5。底物利用实验表明，菌株BA-6具有降解多种苯胺类化合物的能力。发酵培养基优化实验获得一种发酵培养基，活菌量高达3.06×1010 CFU/mL。【结论】苯胺降解菌BA-6对苯胺有较强的降解能力和环境调节能力，在修复苯胺类化合物的生态污染方面有一定的应用前景。
[Background] In recent years, aniline compounds have aggravated the pollution of ecological environment, and biological treatment of aniline wastewater has great development potential and broad application prospect. [Objective] To isolate a strain that can degrade aniline efficiently from activated sludge contaminated by aniline compounds for a long term, and optimize its medium and degradation conditions to provide strain and gene resources for aniline bioremediation. [Methods] The high-efficient degrading bacteria with aniline as its sole carbon and nitrogen source and energy were screened out from the enriched and acclimated strains by plate method. The strain was identified by 16S rRNA gene sequence analysis. The degradation conditions were optimized by the single factor screening test, and the culture conditions were optimized by the orthogonal experiment. [Results] An aniline-degrading strain BA-6 was screened out and identified as Microbacterium sp. The strain removed more than 98% aniline with the initial concentration of 600 mg/L within 24 h. The efficient degradation temperature and pH for the strain BA-6 were 30-37 ℃ and 6.5-7.5, respectively. The substrate utilization test showed that the strain BA-6 had the ability to degrade many aniline compounds. The optimal fermentation medium was obtained, and the viability of the strain was up to 3.06×1010 CFU/mL. [Conclusion] Based on the strong abilities to degrade aniline and adjust to the environment, BA-6 strain has certain application potential in the remediation of ecological pollution of aniline compounds.