Abstract:Microbiology Experiment is a compulsory core course for bioengineering majors. In combination with the school’s “four in one” education concept, the course objectives in line with experimental teaching were established based on Bloom’s taxonomy of educational objectives, and the three-dimensional course objectives of knowledge, ability and ideology and politics were innovatively designed. Under the engineering certification of higher education, the Microbiology Experiment course has been continuously improved for three consecutive sessions; the achievement degree of graduation index has been analyzed and studied; the improvement measures have been proposed; which was worthy of experimental teaching research and promotion.