Abstract:[Background] Pigeon Newcastle disease (ND), caused by pigeon paramyxovirus type Ⅰ (PPMV-1), is a severe infectious disease. However, there is no effective method to control this disease. [Objective] To elucidate the phylogenetic analysis of BJ-C and provide a scientific basis for the prevention and control of pigeon ND. [Methods] Six pairs of specific primers overlapped at the beginning and ending were designed. The genomic cDNA sequence of BJ-C strain was used as the template for amplification and sequencing. The phylogenetic tree of BJ-C strain was established by sequence alignment with the published PPMV-1 and NDV strains on the NCBI website. [Results] The genome length of BJ-C strain was 15 192 nt. BJ-C strain had the closest genetic relationship with PPMV-1/BJ-01/CH strain based on the whole genome analysis, with nucleotide similarity of 99.96% and amino acid similarity of 100%. BJ-C strain shared the same branch with PPMV-1/BJ-01/CH strain, whereas it was far from other ND virus strains such as the LaSota vaccine strain. The phylogenetic tree built based on F gene showed that BJ-C strain was in the same branch with BJP2013 strain isolated in China. The sequence alignment of the hypervariable region (47-420 nt) of F gene showed that the Ⅵb subtype strains including Pigeon/Anhui/2369/2012, Pigeon/Guangdong/GZ288/2013, BJP13, Pigeon/ Zhejiang/2036/2012, PPMV-1/Belgium/11-09620/2011, and BJ-C were in the same clade. [Conclusion] The full-length genome sequence of BJ-C strain was obtained and the strain was grouped to the class Ⅱ Ⅵb type based on phylogenetic analysis. Our results are of theoretical significance for the development of pigeon ND vaccines and other prevention and control methods.
