Abstract:[Background] Blaptica dubia can be used for producing living feed, cosmetics, and healthcare products. Studying the gut bacteria is essential for the breeding of B.dubia and development and utilization of gut bacterial resources. [Objective] To disclose the culturable bacterial species from the gut of B.dubia, and the strains capable of producing digestive enzymes, so as to provide a scientific basis and research materials for research on the influence of gut bacteria on the host and for utilization of the functional bacterial strains. [Methods] The gut bacteria of B.dubia were isolated with the culture method and identified via the methods of morphology and molecular biology. The hydrolysis circle method was employed to screen out the strains capable of producing cellulase, protease, amylase, and lipase, respectively. [Results] A total of 7 species of bacteria belonging to 4 genera were isolated from the gut of B.dubia, including 1 species of Enterococcus, 2 species of Bacillus, 2 species of Serratia, and 2 species of Citrobacter. Ten strains capable of producing digestive enzymes were screened out from the obtained 20 strains. Among them, Bacillus strains D6, D12, and D20 can produce all the 4 digestive enzymes. Serratia strains D3, D7, D9, D11, and D15 had the ability to produce 3 digestive enzymes: cellulase, protease, and lipase. Citrobacter strain D5 and Enterococcus strain D17 can produce cellulase and protease, respectively.[Conclusion] A variety of bacteria in the gut of B.dubia can produce digestive enzymes to help degrade macromolecular nutrients, which may affect the health of host by assisting food digestion. Strains D12, D7, and D11 have the strongest ability to produce cellulase, protease, and lipase, respectively, and are the gut functional strain resources worthy of further development and utilization.
