Abstract:[Background] Two species were discovered during the investigation of macrofungal resources in Hancongling Red Leaf Valley, Dunhua city, Yanbian Korean Autonomous Prefecture, Jilin province, China. [Objective] To determine the taxonomic status of the two species. [Methods] The samples of macrofungi were collected, and their morphology was observed and described in detail. After DNA extraction, the rDNA ITS sequence of each species was determined, and phylogenetic trees were constructed based on maximum likelihood method and Bayesian inference. [Results] The two species were respectively identified as Tulosesus callinus and Russula lakhanpalii, with the morphology consistent with the original description. The phylogenetic analysis confirmed the identification results based on morphology. [Conclusion] R. lakhanpalii is firstly reported in China and T. callinus is a new record species in Jilin province.