真菌N-糖基化系统在异源表达中的研究进展

Research progress of the fungal N-glycosylation system in heterologous expression

摘要:

在近20年来，越来越多的真菌被开发成异源蛋白表达宿主，用来生产各种药用蛋白和酶类。随着对真菌异源表达系统的研究，人们也渐渐意识到真菌N-糖基化系统与高等动物的N-糖基化系统有着明显的区别，这也成为真菌生产高等动物源性糖蛋白的一个技术瓶颈。本文综述了真菌在异源表达糖蛋白工程中其N-糖基化系统的研究进展。包括N-糖基的检测技术和改造策略，并重点介绍了真菌N-糖基化系统与高等动物的N-糖基化系统的差异，以期为日后真菌N-糖基化系统动物源化甚至人源化改造提供参考。

Abstract:

Increasing fungi have been developed as hosts of heterologous expressionfor the production of pharmaceutical proteins and enzymes in the last two decades. With the deepening research on the heterologous expression systems of fungi, people have gradually understood that the fungal N-glycosylation system is visibly different from that of higher animals, which has become a technological obstacle for fungi to produce higher animal-derived glycoproteins. This paper reviews the research progress of fungal N-glycosylation system in heterologous expression. Specifically, we introduced the detection techniques and transformation strategies of N-glycosylation in fungi, and compared the N-glycosylation system between fungi and higher animals, aiming to provide reference for the animalization and even humanization of fungal N-glycosylation system in the future.