肽基载体蛋白结构功能的研究进展

Advances in structure and function of peptidyl carrier protein

摘要:

肽基载体蛋白(peptidyl carrier protein，PCP)是非核糖体肽合成酶(non-ribosomal peptide synthetase，NRPS)的核心结构域。根据NRPS的装配机制，每个模块都至少包含一个PCP，PCP对于非核糖体肽合成中氨基酸残基及多肽在不同催化结构域中的传递起着重要作用，并为氨基酸残基和多肽向模块内其他修饰酶的转移提供一个平台。本文主要对PCP的结构功能、与其他催化结构域的相互作用及重组模块活性降低的问题等方面进行了综述，期望为重组NRPS模块的构建提供理论依据。

Abstract:

Peptidyl carrier protein (PCP) is a core module in non-ribosomal peptide synthetase (NRPS). According to the assembly mechanism, each module of NRPS contains at least one PCP domain. PCP plays a role in the delivery of amino acid residues and polypeptides in different catalytic domains, and it serves as a platform to transfer amino acid residues and polypeptides to other internal modification enzymes. This paper reviews the structure, function, interaction with other catalytic domains, and the reduction of recombinant module activity of PCP, aiming to provide a theoretical basis for the construction of recombinant NRPS modules.